中国药房2012，Vol.23Issue(19)：1764-1766,3.DOI:10.6039/j.issn.1001-0408.2012.19.15
RP-HPLC法测定银天颗粒中主要药效成分的含量
Content Determination of Main Active Components in Yintian Granules by RP-HPLC
摘要
Abstract
OBJECTIVE: To establish methods for the content determination of chlorogenic acid and gasminoidin in Yintian granules. METHODS: RP-HPLC method was adopted. The chromatographic conditions of chlorogenic acid were as follows: Plati-sil-ODS(250 mmx4.6 mm, 5 μm)column was used with mobile phase consisted of acetonitrile-0.2% phosphoric acid(20:80)at a flow rate of 1.0 mL·min‐1.The detection wavelength was set at 326 nm. The column temperature was 25 ℃. The chromatographic conditions of gasminoidin were as follows: Agilent Zorbax SB-C18(250 mm×4.6 mm, 5 um)column was used with mobile phase consisted of acetonitrile-water solution(10:90)at the flow rate of 1.0 mL·min‐1. The detection wavelength was set at 238 nm and column temperature was 25 ℃.. RESULTS: Chlorogenic acid showed a good linear relationship at range of 5.4~ 108.0 μg·mL‐1(r= 0.999 9)with an average recovery of 99.04%(RSD=2.66%,n=6). Gasminoidin showed a good linear relationship at range of 11~ 110 ug·mL‐1(r=0.999 9)with an average recovery of 97.69% (RSD=2.07% ,n=6). CONCLUSION: The methods are specific, reproducible and accurate, and can be used for the quality control of Yintian granules.关键词
银天颗粒/绿原酸/栀子苷/反相高效液相色谱法/含量测定Key words
Yintian granules/ Chlorogenic acid/ Gasminoidin/ RP-HPLC/ Content determination分类
医药卫生引用本文复制引用
张辉,李金玲,王永胜,张聪聪,徐晓玉..RP-HPLC法测定银天颗粒中主要药效成分的含量[J].中国药房,2012,23(19):1764-1766,3.基金项目
国家科技支撑计划项目(2006BAC01A16) （2006BAC01A16）
科技部富民强县专项行动计划项目(国科发农[2008]471号) （国科发农[2008]471号）
