中国药房2012，Vol.23Issue(19)：1772-1774,3.DOI:10.6039/j.issn.1001-0408.2012.19.19
HPLC法测定胡颓子叶中山柰素的含量
Content Determination of Kaempferol in Elaeagnus pungens Leaf by HPLC
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of kaempferol in Elaeagnus pungens leaf. METHODS: HPLC method was adopted. The determination was performed on YMC-Pack ODS-A(250 mm×4.6 mm,5 μm) with mobile phase consisted of acetonitrile-0.3% phosphoric acid (38:62) at flow rate of 1.0 mL·min‐1. The column temperature was set at 25 ℃. The detection wavelength was 366 nm, and the injection volume was 5 μL. RESULTS: The linear range of kaempferol was 0.046~0.552 μg (r=0.999 9) with an average recovery of 99.48% (RSD=1.75% ,n=6). The content of kaempferol from different habitats at different time ranged from 1.87~4.28 mg·g‐1. CONCLUSION: The method is simple, accurate and reproducible, which was suitable for the quality control of E. pungens Leaf.关键词
胡颓子叶/山柰素/高效液相色谱法/含量测定Key words
Elaeagnus pungens/ Kaempferol / HPLC/ Content determination分类
医药卫生引用本文复制引用
李聪,葛月宾,胡婧,应程,王璐瑶,万定荣..HPLC法测定胡颓子叶中山柰素的含量[J].中国药房,2012,23(19):1772-1774,3.基金项目
湖北省自然科学基金计划项目(2007ABA357) （2007ABA357）
中南民族大学中央高校基本科研业务费专项资金项目(CZY11011) （CZY11011）
