中国药房2012，Vol.23Issue(25)：2336-2338,3.DOI:10.6039/j.issn.1001-0408.2012.25.13
α-硫辛酸对顺铂致小鼠耳蜗毛细胞损伤的保护作用的体外研究
Protective Effects of α-lipoic Acid against Cisplatin-induced Cochlea Hair Cells Injury in Mice in Vitro
许涛 1刘双月2
作者信息
- 1. 辽宁医学院科学实验中心,辽宁锦州121000
- 2. 辽宁医学院生理学教研室,辽宁锦州121000
- 折叠
摘要
Abstract
OBJECTIVE: To investigate the protective effect of α-lipoic acid (LA) against cisplatin-induced cochlea hair cells impairment in mice in vitro. METHODS: The cochlea basilar membrane was isolated from postnatal day 3 to 5 Kunming mice cochlea and cultured in culture medium containing 1% bovine serum albumin for 24 hours. Those samples were divided into control group, model group (cisplatin 16 ng·mL-1), drug control group (LA 0.5 mmol·L-1) and LA low and high concentration groups (cisplatin 16 μg·mL-1+LA 0.2, 0.5 mmol·L-1) (n=10). They were cultured in 2 mL new culture medium containing relevant medicine for 24 h respectively. The morphology changes of mice cochlea hair cells were observed. The percentages of internal and external hair cell deficiency were calculated. At the same time, Bax and Bcl-2 expression in cochlea hair cell of mice were detennined. RESULTS: Compared with control group, the cilia of cochlea hair cells in model group were lodged and arranged in disorder. The percentage of missing inner and outer hair cell and Bax expression were significantly increased (P<0.01), while Bcl-2 expression was decreased significantly (P<0.01); compared with model group, the morphological changes of cochlea hair cells in LA groups were obvious alleviated, and the percentage of missing hair cells and Bax expression were significantly decreased (P<0.05 or P< 0.01), while Bcl-2 expression was increased significantly (P<0.05), the improvement of high concentration group was better than low concentration group (P<0.05); there was no significant difference between control group and drug control group (P>0.05). CONCLUSION: This study demonstrates LA might effectively protect cochlea hair cells against cisplatin-induced injury in mice cultured in vitro.关键词
α-硫辛酸/顺铂/离体培养/小鼠/耳蜗/毛细胞/保护作用Key words
α-lipoic acid/ Cisplatin/In vitro culture/ Mice/ Cochlea/ Hair cells/ Protective effects分类
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许涛,刘双月..α-硫辛酸对顺铂致小鼠耳蜗毛细胞损伤的保护作用的体外研究[J].中国药房,2012,23(25):2336-2338,3.
