中国药房2012，Vol.23Issue(25)：2367-2369,3.DOI:10.6039/j.issn.1001-0408.2012.25.24
OP乳化剂荧光猝灭法测定甲壳素胶囊中壳聚糖的含量
Content Determination of Chitosan in Chitin Capsule by Fluorescence Quenching of OP Emulsifier
摘要
Abstract
OBJECTIVE: To establish a method for the content determination of chitosan in Chitin capsule. METHODS: Fluorescence quenching of OP (p-Octyl polyethylene glycol phenyl ether) emulsifier was adopted. The quenching effect of chitosan on OP emulsifier was determined at a wavelength of λex/λwm=226/304 nm in B-R buffer solution (pH 4.5). RESULTS: The linear range of chitosan was 0.20~2.00 mg·L-1 (r=0.999 3). The detection limit was 0.039 mg·L-1. Mean recovery rate was 96.90% (RSD= 3.25%, n=6). CONCLUSION: The method is proved to be convenient, rapid, accurate and reliable. Therefore, it can be applied for the content determination of micro-chitosan in complex samples.关键词
OP乳化剂/荧光猝灭法/壳聚糖/含量测定Key words
OP emulsifier/ Fluorescence quenching/ Chitosan/ Content determination分类
医药卫生引用本文复制引用
白研,苏政权,岑妙兰,郑淑玲..OP乳化剂荧光猝灭法测定甲壳素胶囊中壳聚糖的含量[J].中国药房,2012,23(25):2367-2369,3.基金项目
广东省科技计划项目(2010B030700074) （2010B030700074）
广东省中医药局项目(2009414) （2009414）
中山市科技计划项目(20101H018) （20101H018）
