中国药房2012，Vol.23Issue(27)：2538-2541,4.DOI:10.6039/j.issn.1001-0408.2012.27.16
LC-MS/MS法测定藏药十三味红花丸中羟基红花黄色素A的含量
Content Determination of Hydroxysafflor Yellow A in Tibetan Medicine Shisanwei Honghua Pills by LC-MS/MS
摘要
Abstract
OBJECTIVE: To determine the content of hydroxysafflor yellow A in Tibetan medicine Shisanwei honghua pills. METHODS: The samples were firstly extracted with methanol-water solution (80:20, V/V), and then filtered through 0.22 urn mi-crofilter. The content of hydroxysafflor yellow A was determined by LC-MS/MS. The determination was performed on Agilent Zorb-ax SB-C18(50 mm × 2.1 mm I.D.,3.5μm) column with mobile phase consisted of water (containing 0.1% formic acid)-acetonitrile (containing 0.1% formic acid) with gradient elution. The mass spectrometer was operated in the negative ionization electrospray (ESI) mode using multiple reaction monitoring (MRM) for analysis of hydroxysafflor yellow A at 500℃. The transitions of mlz 611.2→491.2 (hydroxysafflor yellow A) and mlz 268.9→159.0 (genistein) were used to quantity hydroxysafflor yellow A and ge-nistein. RESULTS: The linear range of hydroxysafflor yellow A was 10~l 000 ng-mL-1(r=0.998 9); Average recoveries were 93.8%~106.2%(RSD<4.90% ,n=9). CONCLUSION: The method is rapid, sensitive and accurate, and suitable for the quality control of Tibetan medicine Shisanwei honghua pills.关键词
液相色谱-串联质谱法/羟基红花黄色素A/十三味红花丸/含量测定/藏药Key words
LC-MS/MS/ Hydroxysafflor yellow A/ Shisanwei honghua pills/ Content determination/ Tibetan medicine分类
医药卫生引用本文复制引用
宁宇杉,曾顺泽,彭果,陈铭,王丹..LC-MS/MS法测定藏药十三味红花丸中羟基红花黄色素A的含量[J].中国药房,2012,23(27):2538-2541,4.基金项目
成都医学院院级课题(CYZ07-012) （CYZ07-012）
成都医学院大学生创新实验项目(CX2010014) （CX2010014）
