中国药房2012，Vol.23Issue(31)：2884-2887,4.DOI:10.6039/j.issn.1001-0408.2012.31.02
HPLC法测定兔眼房水中黄芩苷浓度的不确定度评定
Uncertainty Evaluation of the Determination of Baicalin in Rabbit Aqueous Humor by HPLC
摘要
Abstract
OBJECTIVE: To evaluate the uncertainty of the determination of baicalin in rabbit aqueous humor by HPLC. METHODS: The determination process of baicalin in rabbit aqueous humor by HPLC was analyzed. The uncertainty caused by various factors in the whole process of determination, including balancing, solution preparation, calibration fitting and the equipment, were estimated. The uncertainty caused by random effects was evaluated with type A evaluation process and others were by type B. The combined uncertainty was calculated with all the components. RESULTS: The expanded uncertainty for low, medium and high level of baicalin (0.04,0.125,0.25 μg-mL-1) were 0.003, 0.01 and 0.02 μg·mL-1, respectively(P=95% ). CONCLUSION: The method is applicable for the uncertainty evaluation of baicalin determination in rabbit aqueous humor by HPLC. It provides an important reference for the uncertainty evaluation for the determination in complex bio-matrix.关键词
黄芩苷/高效液相色谱法/兔眼房水/不确定度Key words
Baicalin/ HPLC/ Rabbit aqueous humor/ Uncertainty分类
医药卫生引用本文复制引用
张肖宁,侯勇生,霍东风..HPLC法测定兔眼房水中黄芩苷浓度的不确定度评定[J].中国药房,2012,23(31):2884-2887,4.基金项目
黑龙江省中医药、中西医结合重点科研项目(ZHY08-Z72) （ZHY08-Z72）
哈尔滨医科大学第一临床医学院科研基金资助项目(Q08-019) （Q08-019）
