中国药房2012，Vol.23Issue(31)：2920-2921,2.DOI:10.6039/j.issn.1001-0408.2012.31.15
高速逆流色谱法分离黄芩中总黄酮的研究
Separation of Total Flavones from Scutellaria baicalensis by High Speed Counter-current Chromatography
钟锐生 1陈桂红 1李玉兰1
作者信息
- 1. 深圳市宝安区西乡人民医院,广东深圳 518000
- 折叠
摘要
Abstract
OBJECTIVE: To establish a rapid method for separating total flavones from Scutellaria baicalensis. METHODS: High speed counter-current chromatography (HSCCC) was adopted. The extraction solvent composed of dichloromethane-etha-nol-water(7 :3 :1, V/V/V), using upper phase (dichloromethane)as solid phase and lower phase(ethanol-water)as mobile phase at the flow rate of 10 mL·min-1 with revolution speed of 855 r·min-1. The injection volume was 10 mL. The content of total flavones were determined by HPLC. RESULTS: After separation, the content of total flavones reached 98.8% , and 178 mg baicalin was gained from 1 gS. baicalensis. CONCLUSION: The method is simple and easy, and can be used for the separation of the total flavones from S. baicalensis.
关键词
高速逆流色谱法/黄芩/总黄酮/黄芩苷Key words
HSCCC/ Scutellaria baicalensis/ Total flavones/ Baicalin分类
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钟锐生,陈桂红,李玉兰..高速逆流色谱法分离黄芩中总黄酮的研究[J].中国药房,2012,23(31):2920-2921,2.
