中国医科大学学报2012，Vol.41Issue(4)：368-372,5.
皮质发育障碍伴癫痫发作动物模型的制作
Establishment of Rat Model with Disorders of Cortical Development and Epilepsy
贺兴 1张建刚 1张毅 1冯飞 1晏勇1
作者信息
- 1. 重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆400016
- 折叠
摘要
Abstract
Objective To establish the rat model with disorders of cortical developments (DCDs) and epilepsy induced by lithium chloride and pilocarpine following prenatal X-ray radiation. Methods Six pregnant SD rats were randomly divided into experimental group and control group. The experimental group were given X-ray radiation while the control group not. Sixty days after being bom,the male newborn rats were observed in behavior,EEC, brain shape and the pathological changes of cortex and hippocampus. The male newborn rats of the experimental group were injected with lithium chloride and pilocarpine to induce epilepsy. The two groups were compared in terms of incubation period,the total doses of injected drug,Grade V attack rate,behavior and spontaneous seizures. Results Compared with the newborn rats of the control group,those of the experimental group presented with smaller brain and obvious deformity;disordered arrangement of cortical neurons and interrupted CA1 and CA2 in hippocampus with abnormal neuron aggregation,shown by HE;increased activity without spontaneous epilepsy and abnormal discharge in EEG. After the injection of lithium chloride and pilocarpine, the newborn rats of the experimental group had shorter incubation periods (P< 0.05),less doses (P< 0.05) and higher Grade V attack rate (P< 0.05),compared with those of the control group. After recurrent epilepsy,the rats became more irritable and aggressive,and presented with spontaneous seizures during the latent and chronic period and thinner cortex revealed by HE. Conclusion Double intervention by X-ray radiation,lithium chloride and pilocarpine can successfully establish the rat model with diffuse DCDs and result in spontaneous seizures.关键词
X线/皮质发育障碍/癫痫/氯化锂/匹罗卡品Key words
X-ray/ disorders of cortical developments/ epilepsy/ lithium chloride/ pilocarpine分类
医药卫生引用本文复制引用
贺兴,张建刚,张毅,冯飞,晏勇..皮质发育障碍伴癫痫发作动物模型的制作[J].中国医科大学学报,2012,41(4):368-372,5.
