中国医科大学学报2012，Vol.41Issue(6)：485-489,5.
穿山龙总皂苷对胶原诱导性关节炎大鼠关节滑膜组织NF-κB p65活性及STAT3表达的影响
Effects of Total Saponin from Rhizoma Dioscreae Nipponicae on NF-κB p65 Activity and STAT3 Protein Expression in Joint Synovium of CIA Rats
摘要
Abstract
Objective To explore the effects of total saponin from rhizoma dioscreae nipponicae (RDN) on NF-κB p65 activity and STAT3 expression in synovium of collagen-induced arthritis (CIA) rats,and investigate the underlying mechanism about signal transduction pathways of RDN on antiangiogenesis in treating rheumatoid arthritis. Methods A total of 60 rats with successful modeling were randomly divided into 5 groups (n =12):CIA model group,Tripterygium (TP)-treating group (positive control group), RDN-treating group,Diosgerrin-treaiiiig group and Diosgenin-Tablets-tteating group,and established a separate normal control group (n = 12).then the rals in treated groups were laraged with corresponding medicine for 35 days. The NF-κB p65 activity was detected by TransAMTM NF-κB p65 kit. immunohistochemical staining was employed to determine the expression of STAT3 protein in synovium. Results NF-κB p65 activity and the expression of STAT3 protein were more obviously increased in synovium of CIA model group than in normal control group (P < 0.01). The NF-κB p65 activity and the expression of STAT3 protein in RDN-treating,TP-treating,Diosgenin-treating and Diosgenin-tablet-trealing groups were more obviously decreased than in CIA model group (P < 0.01). There was no significant difference between each treated group (P > 0.05). Conclusion Total saponin from KIM can inhibit angiogenesis in synovial of RA,which may be related to the inhibition of NF-κB p65 activity in NF-κB signaling pathway and the down-regulated expression of STAT3 protein which is an important substrate in the JAK-STAT signaling pathway.关键词
胶原诱导性关节炎/穿山龙总皂苷/核转录因子/转录激活因子Key words
collagen-induced arthritis/ total saponin from rhizoma dioscreae nipponicae/ NF-κB p65/ STAT3分类
医药卫生引用本文复制引用
高亚贤,梁秀军,董文娟,肖丽君,宋鸿儒..穿山龙总皂苷对胶原诱导性关节炎大鼠关节滑膜组织NF-κB p65活性及STAT3表达的影响[J].中国医科大学学报,2012,41(6):485-489,5.基金项目
国家自然科学基金项目(30873420) （30873420）
河北省自然科学基金重点项目(C2007000916) （C2007000916）
