中国医科大学学报2012，Vol.41Issue(6)：490-493,4.
选择性剪接变异体的RT-PCR检测及结果分析
Detection and Analysis of Alternative Splicing by RT-PCR
摘要
Abstract
Objective To detect and analyze the alternative splicing of mRNA by RT-PCR. Methods The specific primers were designed targeting the differential DNA sequence of glycogen synthase kinase (CSK) 3β and B-cell translocation gene (BTC) 3 variants,amplified by RT-PCR and analyzed by electrophoresis and DNA cloning sequencing. Results Three bands appeared during the electrophoresis of GSK3β and BTG3 RT-PCR products respectively. On two lop bands of GSK3β, the same product was found by electrophoresis and DNA cloning sequencing. On the three bands of BTG3,the same produce was found after the top (319 bp) and lower (70 bp) bands of BTG3 were amplified by RT-PCR. Conclusion RT-PCR is a simple method to detect the alternative splicing, which is helpful for the evaluation of Western blot results. However, the non-specific bands of PCR might result from the specific nucleic acid sequence of alternative splicing and should be confirmed by DNA sequencing.关键词
选择性剪接/RT-PCRKey words
alternative splicing/ RT-PCR分类
生物科学引用本文复制引用
勾文峰,杨雪,徐小燕,王建平,郑华川..选择性剪接变异体的RT-PCR检测及结果分析[J].中国医科大学学报,2012,41(6):490-493,4.基金项目
国家自然科学基金资助项目(81172371 （）
81001093) （）
