中国医科大学学报2012，Vol.41Issue(6)：522-523,2.
人线粒体转录因子A的表达与前列腺癌细胞顺铂耐药性机制的研究
Expression of Human Mitochondrial Transcription Factor A in Prostate Cancer Cells and Mechanism of Resistance to Cisplatin
韩斌 1白松 1栾岚 2吴斌1
作者信息
- 1. 中国医科大学附属盛京医院泌尿外科,沈阳110004
- 2. 沈阳医学院奉天医院病理科,沈阳110024
- 折叠
摘要
Abstract
Objective To determine the effects of human mitochondria] transcription factor A (TFAM) on the cisplatin resistance of andro-gen independent cell line PC3. Methods Western blot was used to check the expressions of TFAM in the PC3 ceD line and in the cis-platin-resistant cell line PCDP6. After knocking down the TFAM in the PC3, the sensitivity to cisplatin was detected, and IC50 was calculated. Results Compared with its parent cell line PC3, the PCDP6 showed a higher expression of TFAM. The PC3 cells become highly sensitive to cisplatin after knocking down of TFAM by siRNA; at the same time, the expression of suivivin decreased. Conclusion The expression of TFAM improved the resistance to cisplatin in PC3 cell line,and this function of TFAM may be related with the survivin expression.关键词
人线粒体转录因子A/前列腺癌/顺铂/PC3Key words
human mitochondrial transcription factor A/ prostate cancer/ cisplatin/ PC3分类
医药卫生引用本文复制引用
韩斌,白松,栾岚,吴斌..人线粒体转录因子A的表达与前列腺癌细胞顺铂耐药性机制的研究[J].中国医科大学学报,2012,41(6):522-523,2.
