中国油料作物学报2012，Vol.34Issue(2)：129-135,7.
大豆籽粒不同发育时期基因表达谱分析
Gene expression profile of developing soybean seeds
摘要
Abstract
Solexa high - throughput RNA - sequencing were performed on 35DAF ( days after flowering) , 55DAF and 65DAF samples at transcriptional level, while seeds at 15DAF served as control. Totally 9 905 differential expressed unigenes were co — expressed genes. Combined with GO functional annotation and pathway analysis, 12 novel co — expressed unigenes that regulated fatty acid biosynthesis pathway and 6 novel co — expressed unigenes that regulated methionine metabolism were found. qRT — PCR was focused on 12 differentially expressed genes from cDNA materials of 55DAF. Results were consistent with those from Solexa sequencing.关键词
大豆/油脂/蛋白质/脂肪酸/蛋氨酸/差异表达基因Key words
Soybean/ Oil/ Protein/ Fatty acid/ Methionine/ Differential expressed gene分类
农业科技引用本文复制引用
陈欢,陈光,杨美英,王真慧,孙旸,王刚,魏玮,关瑜,王法微,李海燕..大豆籽粒不同发育时期基因表达谱分析[J].中国油料作物学报,2012,34(2):129-135,7.基金项目
科技部863项目(2011AA1000691008) （2011AA1000691008）
国家转基因生物新品种培育重大专项(2010ZX08010-002) （2010ZX08010-002）
吉林省优秀创新团队项目(20111815) （20111815）
