中国油料作物学报2012，Vol.34Issue(2)：136-141,6.
利用青海大黄油菜和芥蓝合成大粒甘蓝型油菜
Large seed resynthesized Brassica napus from hybrid of Brassica rapa and Brassica oleracea var alboglabra
摘要
Abstract
Inter -species hybridization between Brassica rapa cv Qinghai Dahuang (QD, 2n =20, AA) and several B. oleracea var alboglabra L. (CC, 2n = 18 ) was made to artificially synthesize novel Brassica napus through embryo rescue, ovary culture and chromosome doubling. 69 embryos were produced in QD X B. oleracea var alboglabra L. crosses, but none of them was produced from the reciprocal crosses through embryo rescue. In QD x Mid -late -flowering kale cross,24 hybrid embryos were survieved through ovary culture. The embryo surviving rate from ovary culture on 3 types of medium ( MS,1/2MS and MS +0.2mg/L 6 - BA) were 15.5% ,8. 9% and 4.4% respectively. Mature embryo could not be obtained from the differentiation medium (MS + 1. 5mg/L 6 - BA + 0.25 mg/L NAA). The result of chromosome doubling showed that doubling ratio of plants on culture medium with 100mg/L colchicine was the highest, which varied from 59. 4% to 86. 4%. The SSR analysis demonstrated the plants examined were true F, hybrids, from which neither novel bands nor absent bands were observed. The hybrids between QD x B. oleracea var alboglahra L. had an intermediate morphology and greater average 1 000 - seed weight of novel Brassica napus varying from 5.48g to 7.45g, which were significantly higher than B. napus cv Qingy-ou 14 and Zhongshuang 4.关键词
人工合成/甘蓝型油菜/SSR分析/千粒重Key words
Artificially synthesize/ Brassica napus/ SSR analysis/ 1 000 -seed weight分类
农业科技引用本文复制引用
富贵,赵志刚,杜德志..利用青海大黄油菜和芥蓝合成大粒甘蓝型油菜[J].中国油料作物学报,2012,34(2):136-141,6.基金项目
国家自然科学基金(30960200) （30960200）
青海省应用基础研究(2011-Z-701) （2011-Z-701）
国家“973”前期研究(2011CB111508) （2011CB111508）
