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富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响

桂恒 张培培 华小梅 彭欣 孔令如 陈丰 戚金亮 喻德跃 杨永华

中国油料作物学报2012,Vol.34Issue(2):181-187,7.
中国油料作物学报2012,Vol.34Issue(2):181-187,7.

富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响

Effect of transgenic soybean with sulfur - rich amino acids on soil microbial population structure

桂恒 1张培培 1华小梅 2彭欣 1孔令如 1陈丰 1戚金亮 1喻德跃 3杨永华1

作者信息

  • 1. 南京大学生命科学学院,医药生物技术国家重点实验室,南京大学-南京林业大学植物分子生物学联合研究所,江苏南京210093
  • 2. 环境保护部南京环境科学研究所,江苏南京210042
  • 3. 南京农业大学国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏南京,210095
  • 折叠

摘要

Abstract

Effects of transgenic soybeans on microbial population structure of rhizosphere soil were investigated under field conditions based on phospholipid fatty acid (PLFA) method. Two groups of transgenic soybeans with sulfur - rich amino acids were used. Results showed that soil microbial population in rhizosphere of transgenic lines changed significantly in both group A and B. 15 specific PLFAs were identified in group A, and 13 in group B. Correlation analysis found that microbial characterization of group A and the total concentration of PLFA' s of the transgenic line OE -8 were significantly higher than non -transgenic line, and so were 17-4 line and 57 line in group B. The dominant fifteen PLFA mown plots were 16: 0, 10Mel8: 0 and 18: 1ω7c in group A, and dominant thirteen PLFA were 15: 0, 16: 0 and 14Mel6: 0 in group B. The results suggested that the microbial population structure changed after planting transgenic soybeans.

关键词

转基因大豆/土壤微生物群落/磷脂脂肪酸

Key words

Transgenic soybean/ Soil microorganism population/ PLFA (phospholipid fatty acid)

分类

农业科技

引用本文复制引用

桂恒,张培培,华小梅,彭欣,孔令如,陈丰,戚金亮,喻德跃,杨永华..富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响[J].中国油料作物学报,2012,34(2):181-187,7.

基金项目

国家重大专项(2011ZX08011-003) (2011ZX08011-003)

国家自然科学基金(30971871,40771107) (30971871,40771107)

教育部创新团队项目(IRT1020) (IRT1020)

教育部高校科技创新工程重大项目培育资金(707027) (707027)

中国油料作物学报

OA北大核心CSCDCSTPCD

1007-9084

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