中国医学科学院学报2012，Vol.34Issue(4)：384-389,6.DOI:10.3881/j.issn.1000-503X.2012.04.014
铜绿假单胞菌对支气管扩张患者气道中基质金属蛋白酶-9和金属蛋白酶组织抑制因子-1表达的影响
Effect of Pseudomonas Aeruginosa on the Expressions of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in the Airway of Bronchiectasis Patients
摘要
Abstract
Objective To study the expressions of matrix metalloproteinase-9 (MMP^) and tissue inhibitor of metalloproteinase-1 ( TIMP-1 ) in the airway of bronchiectasis ( BE ) patients, and evaluate the effect of pseudomonas aeruginosa (PAE) on the expression of TIMP-1 and MMP-9. Methods In this case-control study, subjects were divided into BE group and control group, and the BE group was further divided into PAE group and other bacteria group based on the culture results of bronchoalveolar lavage fluid ( BALF) . BALF was obtained by bronchoscopy, the expressions of MMP-9 and TIMP-1 were determined by ELISA, then the ratio of TIMP-l/MMP-9 was calculated. Furthermore, the tissue of bronchic endomembrane was obtained by transbronchial biopsy and the expressions of MMP-9 and TIMP-1 were determined using immunohistochemi-cal method. Results The levels of MMP-9 in the BALF of PAE group and other bacteria group were significantly higher than that in control group (P =0. 0000 both) , and the expressions of MMP-9 in bronchic endomembrane of PAE group and other bacteria group were also significantly higher {P =0. 0421 and 0. 0003 , respectively). The level of TIMP-1 in BALF of PAE group was significantly lower than that in other bacteria group (P = 0. 0324). The ratio of TIMP-l/MMP-9 in BALF of BE group was significantly lower than that in control group ( P = 0. 0000) , and this ratio of PAE group was significantly lower than those in both other bacteria group and control group (P = 0. 0026 and 0. 0000, respectively). Conclusion PAE infection in BE patients can suppress the expression of TIMP-1 and stimulate the expression of MMP-9 , and thus make the disease even worse.关键词
铜绿假单胞菌/支气管扩张/基质金属蛋白酶-9/金属蛋白酶组织抑制因子-1Key words
pseudomonas aeruginosa/bronchiectasis/matrix metalloproteinase-9 /tissue inhibitor of metalloproteinase-1分类
医药卫生引用本文复制引用
金前,黄飞华,周颖,张影清,柴秀娟..铜绿假单胞菌对支气管扩张患者气道中基质金属蛋白酶-9和金属蛋白酶组织抑制因子-1表达的影响[J].中国医学科学院学报,2012,34(4):384-389,6.基金项目
浙江省医药卫生科学研究基金(2008B033) （2008B033）
