中国肿瘤生物治疗杂志2012，Vol.19Issue(4)：348-354,7.DOI:10.3872/j.issn.1007-385X.2012.04.002
干扰素-α对嗜酸性粒细胞抗白血病免疫效应的增强作用
Interferon-α enhances anti-leukemia immunological effect of eosinophils granulocytes
摘要
Abstract
Objective; To observe the immunological enhancement effect of interferon-a (IFN-a) on anti-leukemia activity of eosinophils granulocytes (EOSs) in patients with chronic myelogenous leukemia (CML) after hydroxycarbmide (OHU) therapy, and to investigate the related immunologic mechanism. Methods; Forty-six BCR-ABL positive CML patients (from Jan. 2010 to Feb. 2012) admitted in the 148' Hospital of PLA were included in this study, and were divided into OHU group (20 cases) and OHC combined IFN-a group (26 cases) with a balance in sex ratio and age range. Meanwhile, specimens of 30 healthy volunteers from the Regular Physical Examination Center of the hospital were collected as control. ELJSA was used to determine the concentrations of cytokines IL-6, IL-12, IL-17 and IFN-7 in serum of the CML patients. Cytochemistry staining was used to observe the peroxydase ( POX ) expression of immunocyte in bone marrow. Immunofluorescence (IF) staining was used to observe cytokines IL-12 and IL-17A expression levels as well as the expression of mannose receptor ( MR). Results; The serum concentrations of IL-6, IL-12 and IL-17 were increased significantly in CML patients compared with the healthy control group [(116. 13 ± 15. 16) vs (90. 98 ± 12. 32) pg/ml; (189. 26 ± 22.14) vs (96.60±4.92) pg/ml; (34.42 ±2.16) vs (23.74 ±1.36) pg/ml, P<0.05]. After OHU treatment, the serum concentrations of IL-6, IL-12 and IL-17 in the CML patients decreased to (87. 14 ± 13. 37) , (60. 22 ±20. 16) and (17.03 ±2. 16) pg/ml, respectively (P<0.05). Compared with the OHU treatment group, the serum IL-6, IL-12 and IL-17 concentrations in the OHU combined IFN-a treatment group were significantly up-regulated [ ( 122. 04 ± 10. 25) , ( 101. 12 ±27. 16) and (40. 16 ±4. 11) pg/ml, P <0. 05 or P <0.01 ]. EOS in bone marrow of CML patients expressed IL-12, IL-17A and MR. OHU treatment combined with IFN-a could decrease immunosuppressive effect of OHU therapy, the quantity of EOS with positive IL-12, IL-17A and MR expressions was increased obviously, and the POX activity of EOS was also increased. Conclusion; EOS can secrete cytokines IL-12 and IL-17 and express MR. OHU treatment combined with IFN-a can enhance the anti-leukemia immunological effect of EOS and decrease the immunosuppressive effect of OHU therapy on EOS.关键词
慢性粒细胞白血病/嗜酸性粒细胞/干扰素-α/羟基脲/IL-12/IL-17A/甘露糖受体Key words
chronic myelogenous leukemia/ eosinophils granulocyte/ interferon-a/ hydroxycarbmide/ IL-12/ IL-17A/ mannose receptor分类
医药卫生引用本文复制引用
孙黎飞,韩冰,马向杉,吴强强,郝红峰,王冬青..干扰素-α对嗜酸性粒细胞抗白血病免疫效应的增强作用[J].中国肿瘤生物治疗杂志,2012,19(4):348-354,7.基金项目
济南军区“十二五”科技发展计划重点支持项目(No.JN11LO17). （No.JN11LO17）
