中国肿瘤生物治疗杂志2012，Vol.19Issue(4)：392-396,5.DOI:10.3872/j.issn.1007-385X.2012.04.009
siRNA沉默livin的表达对人结肠癌HT-29细胞增殖、凋亡及侵袭的影响
Effects of siRNA silencing livin expression on proliferation, apoptosis and invasion of human colon cancer cell line HT-29
摘要
Abstract
Objective: To explore the effects of small interference RNA (siRNA) targeting livin on the proliferation, apoptosis and invasion of human colon cancer cell line HT-29. Methods; Chemically synthetic double-strand siRNA targeting livin (livin-siRNA) was transfected into HT-29 cells, and then RT-PCR and Western blotting were used to detect the expression of livin mRNA and protein in HT-29 cells. MTT assay was performed to analyze the proliferation of HT-29 cells. The cell apoptosis and cell cycle distribution were analyzed by flow cytometry. The invasion assay and caspase-3 detective kit were used to detect the change of invasion and caspase-3 activity in HT-29 cells. Results: Forty-eight hours after transfection, there was a significant decrease in the expressions of both livin mRNA ( 0. 073 ± 0. 007 vs 0. 395 ± 0- 082, 0.423 ± 0. 025, 0. 418 ± 0. 032, P < 0. 05) and livin protein (0. 106 ± 0. 003 vs 0. 456 ± 0. 065, 0. 473 ± 0.078,0.491 ±0.045, P<0.05) in the livin-siRNA group, compared with the blank and negative control and liposome groups. Ninety-six hours after transfection, the growth of HT-29 cells in the livin-siRNA group was significantly lower than that in the control and liposome groups (0.564 ±0. 102 vs 0.833 ±0. 127, 0.860 ±0. 153 ,P <0. 05) , and the rate of apoptosis was obviously increased ( [16. 5 ±2.8] % vs [2.4 ±0.5]% , [3.7 ±1.0] % , P<0. 05). The invasion assay demonstrated that the number of the migration cells was lower in the livin-siRNA group than in the control and liposome groups (31.3 ±4. 5 vs 101. 3 ± 8. 6, 97. 4 ± 7. 8, P < 0.05 ). The activity of caspase-3 in the livin-siRNA group was decreased compared with that in the control group (0. 160 ±0.023 vs 0. 347 ± 0.058 , P <0.05 ). Conclusion: The siRNA silencing livin expression in HT-29 cells can suppress the proliferation, induce the apoptosis and inhibit the invasion of HT-29 cells.关键词
结肠癌/livin/小干扰RNA/基因治疗Key words
colon cancer/ livin/ small interference RNA/ gene therapy分类
医药卫生引用本文复制引用
何文静,黎军和,赵清梅,熊建萍,彭小东..siRNA沉默livin的表达对人结肠癌HT-29细胞增殖、凋亡及侵袭的影响[J].中国肿瘤生物治疗杂志,2012,19(4):392-396,5.基金项目
国家自然科学基金资助项目(No.30960440). （No.30960440）
