中国肿瘤生物治疗杂志2012，Vol.19Issue(4)：408-413,6.DOI:10.3872/j.issn.1007-385X.2012.04.012
聚酰胺树形分子-脂质体介导survivin反义寡核苷酸诱导肝癌细胞的凋亡
Survivin antisense oligonucleotide mediated by polyamidoaminedendrimer liposome induces apoptosis of hepatic cancer cells
摘要
Abstract
Objective: To investigate the possibility of polyamidoaminedendrimer ( PAMAM ) -liposome for survivin antisense oligonucleotide (survivin-asODN) delivery system and explore the effects of PAMAM-liposome-survivin-asODN on survivin expression and apoptosis of human hepatic cancer cell line SMMC-7721. Methods; The liposome modified PAMAM (PAMAM-liposome) was synthesized with liposome and PAMAM. Survivin-asODN was combined with the PAMAM-liposome or PAMAM to form PAMAM-liposome-survivin-asODN or PAMAM-survivin-asODN complexes. The shape and size of the two complexes were observed under a transmission electron microscope and their zeta potentials were measured with a zeta analytical tool. The encapsulating efficiency and DNA loading level were determined by ultraviolet spectropho-tometer using a centrifuging method. PAMAM-liposome-survivin-asODN and PAMAM-survivin-asODN were transfected into SMMC-7721 cells, and the transfection efficiency was measured. The protein expression of survivin in SMMC-7721 cells was measured by Western blotting, and the apoptosis of SMMC-7721 cells was assessed by flow cytometry. Results: PAM-AM-liposome, PAMAM-liposome-survivin-asODN and PAMAM-survivin-asODN were successfully established. No significant difference appeared in diameter between PAMAM-liposome-survivin-asODN and PAMAM-survivin-asODN ([ 189.33 ± 15.42] vs [ 181.83 ± 13.67] nm, P >0.05), as well as the encapsulating efficiency and drug loading level, but the zeta potential of PAMAM-liposome-survivin-asODN was higher than that of PAMAM-survivin-asODN ([42.83 ±7. 14] vs [32.33 ±5.57] mV, P<0.05). The transfection efficiency of PAMAM-liposome-survivin-asODN was higher than that of PAMAM-survivin-asODN ([73. 33 ±9.29]% vs [60.67 ±7. 81 ]% , P<0.05] in SMMC-7721 cells. The expression of survivin protein in SMMC-7721 cells of PAMAM-liposome-survivin-asODN group was less than that of PAMAM-survivin-asODN group (24. 67 ± 11.74 vs 43.17 ± 11. 63, P < 0. 05 ) , while the apoptosis rate was higher than that of PAMAM-survivin-asODN ([73.31 ±12.59]% vs [52.67 ±12.19]% , P<0.05). Conclusion; The PAM-AM-liposome can delivery survivin-asODN into SMMC-7721 cells effectively and induce SMMC-7721 cell apoptosis.关键词
聚酰胺-胺型树枝状高聚合物-脂质体/反义寡核苷酸/肝癌/survivinKey words
polyamidoaminedendrimer liposome/ antisense oligonucleotide/ hepatic cancer/ survivin分类
医药卫生引用本文复制引用
厉周,方素珍,韩帅,崔大祥,李强,蔡寨,朱卉娟,黄宗海..聚酰胺树形分子-脂质体介导survivin反义寡核苷酸诱导肝癌细胞的凋亡[J].中国肿瘤生物治疗杂志,2012,19(4):408-413,6.基金项目
广东省自然科学基金资助项目(No.9151051501000071) （No.9151051501000071）
