首页|期刊导航|中华医学杂志（英文版）|Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data
中华医学杂志（英文版）2012，Vol.125Issue(13)：2284-2288,5.DOI:10.3760/cma.j.issn.0366-6999.2012.13.008
Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data
Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data
摘要
Abstract
Background Multi-locus sequence typing (MLST) is widely used to explore the population structure of numerous bacterial pathogens.However,for genotypically-restricted pathogens,the sensitivity of MLST is limited by a paucity of variation within selected loci.For Bartonella henselae (B.henselae),although the MLST scheme currently used has been proven useful in defining the overall population structure of the species,its reliability for the accurate delineation of closely-related sequence types,between which allelic variation is usually limited to,at most,one or two nucleotide polymorphisms.Exploitation of high-throughput sequencing data allows a more informed selection of MLST loci and thus,potentially,a means of enhancing the sensitivity of the schemes they comprise.Methods We carried out SOLiD resequencing on 12 representative B.henselae isolates and explored these data using single nucleotide polymorphism (SNP) analysis.We determined the number and distribution of SNPs in the genes targeted by the established MLST scheme and modified the position of loci within these genes to capture as much genetic variation as possible.Results Using genome-wide SNP data,we found the distribution of SNPs within each open reading frame (ORF) of MLST loci,which were not represented by the established B.henselae MLST scheme.We then modified the position of loci in the MLST scheme to better reflect the polymorphism in the ORF as a whole.The use of amended loci in this scheme allowed previously indistinguishable ST1 strains to be differentiated.However,the diversity of B.henselae was still rare in China.Conclusions Our study demonstrates the use of SNP analysis to facilitate the selection of MLST loci to augment the currently-described scheme for B.henselae.And the diversity among B.henselae strains in China is markedly less than that observed in B.henselae populations elsewhere in the world.关键词
Bartonella henselae/multilocus sequence typing/single-nucleotide polymorphismKey words
Bartonella henselae/multilocus sequence typing/single-nucleotide polymorphism引用本文复制引用
ZHAO Fan,Gemma Chaloner,Alistair Darby,SONG Xiu-ping,LI Dong-mei,Richard Birtles,LIU Qi-yong..Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLiD sequence data[J].中华医学杂志（英文版）,2012,125(13):2284-2288,5.基金项目
This research was supported by a grant of the National Science and Technology Major Project of the Ministry of Science andTechnology of China (No. 2008ZX10004-010 （）
No.2008ZX10004-008 （）
No.2012ZX10004-215). （）
