首页|期刊导航|中华医学杂志（英文版）|Endothelial progenitor cell transplantation ameliorates elastin breakdown in a Kawasaki disease mouse model
中华医学杂志（英文版）2012，Vol.125Issue(13)：2295-2301,7.DOI:10.3760/cma.j.issn.0366-6999.2012.13.010
Endothelial progenitor cell transplantation ameliorates elastin breakdown in a Kawasaki disease mouse model
Endothelial progenitor cell transplantation ameliorates elastin breakdown in a Kawasaki disease mouse model
摘要
Abstract
Background Coronary artery damage from Kawasaki disease (KD) is closely linked to the dysfunction of endothelial progenitor cells (EPCs).The aim of the present study was to evaluate the therapeutic effect of EPCs transplantation in KD model.Methods Lactobacillus casei cell wall extract (LCWE)-induced KD model in C57BL/6 mice was established.The model mice were injected intravenously with bone marrow-derived in vitro expanded EPCs.Histological evaluation,number of circulating EPCs and the function of bone marrow EPCs were examined at day 56.Results Inflammation was found around the coronary artery of the model mice after 14 days,Elastin breakdown was observed after 56 days.CM-Dil labeled EPCs incorporated into vessel repairing foci was found.At day 56,the number of peripheral EPCs in the KD model group was lower than in EPCs transplanted and control group.The functional index of bone marrow EPCs from the KD model group decreased in proliferation,adhesion and migration.Increased number of circulating EPCs and improved function were observed on the EPCs transplanted group compared with model group.Conclusion Exogenously administered EPCs,which represent a novel strategy could prevent the dysfunction of EPCs,accelerate the repair of coronary artery endothelium lesion and decrease the occurrence of aneurysm.关键词
Kawasaki disease/endothelial progenitor cell/transplantation/mouse modelKey words
Kawasaki disease/endothelial progenitor cell/transplantation/mouse model引用本文复制引用
CHEN Zhi,DU Zhong-dong,LIU Jun-feng,LU Dun-xiang,LI Li,GUAN Yun-qian,WAN Sui-gui..Endothelial progenitor cell transplantation ameliorates elastin breakdown in a Kawasaki disease mouse model[J].中华医学杂志（英文版）,2012,125(13):2295-2301,7.基金项目
This study was supported by the grants from National Natural Science Foundation of China (No.30973238),Beijing Natural Science Foundation (No.7092032),Key Research Project of Beijing Natural Science Foundation (B)/Beijing Education Committee (No.KZ201010025024) and Beijing “215” Medical Professional Project Fund (No.2009-3-38). （No.30973238）
