中华医学杂志（英文版）2012，Vol.125Issue(15)：2741-2745,5.DOI:10.3760/cma.j.issn.0366-6999.2012.15.016
Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene
Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene
HAO Yi-xin 1HE Zheng-wen 2ZHU Jian-hua 1SHEN Qian 2SUN Jun-zhong 1DU Nan 1XIAO Wen-hua1
作者信息
- 1. Department of Oncology, First Affiliated Hospital of the People's Liberation Army General Hospital, Beijing 100048, China
- 2. Department of Laboratory Diagnosis. Changhai Hospital, Second Military Medical University, Shanghai 200433, China
- 折叠
摘要
Abstract
Background Over-expression of P-glycoprotein (P-gp),encoded by the MDR1 gene,confers multidrug resistance (MDR) in renal cell carcinoma (RCC) and is a major reason for unsuccessful chemotherapy.This study aimed to determine the effct of RNA interference (RNAi) on the reversal of MDR in human RCC.Methods We designed and selected one short hairpin RNA (shRNA) targeting MDR1 gone,which is stably expressed from integrated plasmid and transfected by lentivirus fluid in human RCC A498 call.Results The MDRl-targeted RNAi resulted in decreased MDR1 gone mRNA level (P ＜0.001).almost abolished Pogp expression and reversed MDR to different chemotherapy drugs in the RCC A4g8 cell line.Conclusion MDR could be reversed by RNAi in human RCC A498 cell line,which may be used for clinical application in future.关键词
multidrug resistance/P-glycoprotein/short hairpin RNA/renal cell carcinomaKey words
multidrug resistance/P-glycoprotein/short hairpin RNA/renal cell carcinoma引用本文复制引用
HAO Yi-xin,HE Zheng-wen,ZHU Jian-hua,SHEN Qian,SUN Jun-zhong,DU Nan,XIAO Wen-hua..Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene[J].中华医学杂志（英文版）,2012,125(15):2741-2745,5.
