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Proteomic analysis for detecting serum biomarkers related to smoking in humans

XIAO Dan ZHAO Li-juan CHU Shui-lian JING Hang WANG Chen

中华医学杂志（英文版）2012，Vol.125Issue(17)：3027-3032,6.

中华医学杂志（英文版）2012，Vol.125Issue(17)：3027-3032,6.DOI:10.3760/cma.j.issn.0366-6999.2012.17.011

Proteomic analysis for detecting serum biomarkers related to smoking in humans

XIAO Dan ¹ZHAO Li-juan ²CHU Shui-lian ³JING Hang ⁴WANG Chen¹

作者信息

1. Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders,Beijing Institute of Respiratory Medicine,WHO Collaborating Center for Tobacco or Health,Beijing 100020,China
2. Department of Respiratory Diseases,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China
3. Department of Respiratory Diseases,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China
4. Department of Respiratory Epidemiology Research,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China
折叠

摘要

Abstract

Background Smoking is the leading cause of death in the world.This study focused on the difference of the serum proteomic profiling between healthy smokers and nonsmokers in order to find smoking-specific serum biomarkers.Methods Pattern-based proteomic profiling of 100 serum samples (from 50 Chinese male smokers and 50 matched nonsmokers) was performed through magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (MALDI-TOF-MS) and resulting data were statistically analyzed by Ciphergen ProteinChip software 3.0.2.Results We found 72 serum peaks were significantly different between smokers and nonsmokers (P ＜0.05).Marker peaks of mass-to-charge ratio (m/z) 3159.13,7561.03 and 9407.32 were smoking-specific.Conclusion The preliminary data suggested that smoking-specific serum biomarkers could be detected in humans.

关键词

smoking/ serum/ biological markers/ proteomics

Key words

smoking/ serum/ biological markers/ proteomics

引用本文复制引用

XIAO Dan,ZHAO Li-juan,CHU Shui-lian,JING Hang,WANG Chen..Proteomic analysis for detecting serum biomarkers related to smoking in humans[J].中华医学杂志（英文版）,2012,125(17):3027-3032,6.

基金项目

This work was supported by the Beijing Nova Program (No.2006B04). （No.2006B04）

中华医学杂志（英文版）

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ISSN：0366-6999

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