肿瘤防治研究2012，Vol.39Issue(7)：787-792,6.DOI:10.3971/j.issn.1000-8578.2012.07.007
胶质瘤模型单剂量全脑放疗后血脑屏障开放的时间阈及紧密连接变化的研究
Study of Time-Domain of Blood-brain Barrier Opened and Tight Junction Changed in Glioma Models after Whole-brain Irradiated with Single Dose
摘要
Abstract
Objective To investigate the change in blood-brain barrier (BBB) permeability and ultrastruc-ture of microvessle in C6 glioma and the tight junction protein expression of claudin-5.which were whole-brain irradiated with 15 Gy,and to explore the time-domain of BBB opened. Methods Sixty tumor-bearing rats (17thday after inoculation) were whole-brain irradiated with 15 Gy,and 10 rats were severally sacrificed at 24 h,3 d,7 d,14 d,21 d and 28 d after irradiation,5 rats were using transmission electron microscopy of exogenous lanthanum nitrate (La3+ )-tracing to observe the BBB permeability of the tissues within 2 mm to the border of the tumor and using immunohistochemicai method to detect the expression of claudin-5.respectively. Results (l)At 24 h,3 d after exposure,La3+ obviously exuded to the basement membrane(BM),extra-vascular,the space inside the brain tissue and cells,a little remaining in the endo-vascular. At 7 d after exposure,La3+ obviously exuded to the basement membrane and brain tissue space less than that in the previous days. At 14d after exposure, La3+ exuded to the local basement membrane. At 21 d,28 d after exposure,La3+ exudation was disappeared. Statistical analysis showed that 24 h,3 d> 7 d>14 d>21 d,28 d (P<0. 05). (2)claudin-5 expression was mild at 24 h and 3 d after irradiation, showed debris-like expression, claudin-5 expression was light and moderate at 7 d after exposure, combined with deformation and continuous expression. It was moderate expression 14 d after exposure, showed vascular distortion and deformation,but was still continuous state. It was medium-intensity expression at 21 d and 28 d after irradiation,appeared to express both a row and debris. Statistical analysis showed that 24 h,3 d<7 d<14 d<21 d,28 d(P<0. 05). Conclusion BBB is opened and gradually resuming 2 weeks after irradiated with 15Gy in C6 glioma which is in time-dependent manner.关键词
C6脑胶质瘤/血-脑屏障/通透性/超微结构/claudin-5Key words
C6 glioma: Irradiation/Blood-brain barrier(BBB) /Ulrrastucture/claudin-5分类
医药卫生引用本文复制引用
李蓉,刘国龙,关明媚,牛道立,易炜,孙健聪..胶质瘤模型单剂量全脑放疗后血脑屏障开放的时间阈及紧密连接变化的研究[J].肿瘤防治研究,2012,39(7):787-792,6.基金项目
广东省科技计划资助项目 （）
