中山大学学报（自然科学版）2012，Vol.51Issue(4)：92-96,5.
几种水蛭抗凝血物质提取及活性分析
Anticoagulant Active Substances Extraction and Anti-Thrombin Activity Analysis of Several Species of Leeches
摘要
Abstract
The anticoagulant activity in vitro of three different sample isolated from the leech, Hirudinar ia manillensis with three different extracting methods was studied, and utilized the optimal extracting process, the anti-thrombin bioactivity of five species of leeches were analyzed, and compared the anti thrombin bioactivity of three species of wild H. manillensis from different geographical distribution and two species of cultured H. manillensis reared with different diets. The results showed that among them, the extracts produced by acetone precipitation had the highest yield, and also displayed the stronger anti coagulant activity tested than others ( P < 0. 05 ). The test displayed three species of non-bloodsucking leeches ( Whitmania pigra, Whitmania acranulata and Whitmania laevis) had no or lower anti-thrombin activity, and the two species of blood-sucking leeches (H. manillensis and Hinuio nipponia) had higher anli-thrombin activity, and that the anti-thrombin activity of H. manilhnsis was higher than Hirudo nip ponia significantly (P <0. 01). There was no significant difference of inhibitory activity to thrombin between wild and cultured H. manillensis (P > 0. 05). In conclusion, the leech of H. manillensis had high anti-thrombin bioactivity in vitro, and the artificial breeding had no adverse effect on its quality and anti coagulant activity.关键词
水蛭/菲牛蛭/凝血酶/抗凝作用/提取液Key words
leech/Hirudinaria manillensis/thrombin/anticoagulant activity/extraction分类
生物科学引用本文复制引用
张彬,汪波,龚元,于翔,吕军仪..几种水蛭抗凝血物质提取及活性分析[J].中山大学学报（自然科学版）,2012,51(4):92-96,5.基金项目
广州市海珠区科技计划资助项目(2006AA10A402) （2006AA10A402）
辽宁省海洋与渔业厅科技基金资助项目(200903) （200903）
