中山大学学报（自然科学版）2012，Vol.51Issue(4)：106-111,133,7.
俄罗斯鲟雌核发育诱导及其后代的微卫星分析
Microsatellite Marker Analysis of Gynogenesis by Induction in Russian Sturgeon(Acipenser gueldenstaedtii)
摘要
Abstract
Gynogenesis is the production of an embryo from an egg after penetration by a spermatozoon that does not contribute genetic material. The process of gynogenesis involves two steps; firstly, the sperm is inactivated by UV irradiation; secondly, the meiotic diploids are made by restraint of the extru sion of the secondary polar body. Gynogenesis was induced in two groups of Russian sturgeon (Acipenser gueldenstaedtii) by cool shock and heat shock respectively. Cool shock ( Ml) and heat shock (M2) gy-nogensis offspring were gained separately. Six polymorphic microsatellite markers were used to investigate genotype by PCR amplification. Twenty fries in each group including gynogenetic families Ml , control group and their parents and forty fries in gynogenetic families M2 were chose to be analyzed . The aver age expected heteroaygosity (Ht) , allele number (A) and allele frequencies (P) in the cool shock line was 0. 591, 6. 0 and 0. 100 ~0. 708 respectively. In the heat shock line, the average expected heteroay gosity, allele number and allele frequencies was 0. 687, 5. 5 and 0.006 ~ 0. 774 respectively. Special microsatellite bands of male parent were used as diagnostic markers to analyze the hybrid of gynogenesis offspring. The results showed that 40 % in cool shock genogenesis offspring and 27. 5% in heat shock ge-nogenesis offspring were hybrid respectively. And, it is consistent with phenotypic identification results. Haploid individuals were identified in 10% of cook shock group and 15% of heat shock group. Complete gynogenetic progenies were found only in heat shock group, and they were just about 22. 5%. Gene re combination in different levels were found in other offspring except haploid, hybrid and gynogenisis off spring of two gynogenetic lines.关键词
俄罗斯鲟Acipenser gueldenstaedtii/雌核发育/微卫星Key words
Acipenser gueldenstaedtii/gynogenesis/microsatellite分类
生物学引用本文复制引用
姜彦超,苏建通,董颖,胡红霞..俄罗斯鲟雌核发育诱导及其后代的微卫星分析[J].中山大学学报（自然科学版）,2012,51(4):106-111,133,7.基金项目
北京市科技计划资助项目(D121100003712002) （D121100003712002）
农业公益性行业科研专项基金资助项目(201003055-05) （201003055-05）
国家星火计划资助项目(2010GA600005) （2010GA600005）
