中山大学学报（医学科学版）2012，Vol.33Issue(4)：440-443,448,5.
自噬在血管紧张素Ⅱ诱导心肌细胞肥大中的作用
Role of Autophagy in Angiotensin Ⅱ Induced Cardiac Hypertrophy
摘要
Abstract
[Objective] To investigate the role of autophagy in cardiac hypertrophy induced by angiotensin Ⅱ (Ang Ⅱ ). [Methods] Cardiac hypertrophy was induced by Ang II incultured neonatal rat cardiomyocytes. Morphology and expression of hypertrophy marker atrial natriuretic peptide (ANP) were detected by optical microscope and real time-PCR. Autophagic marker, LC3 level was tested by Western blotting.[Results]Ang Ⅱ increased volume of cardiomyocyte, and up regulated RNA expression of ANP. Ang Ⅱ inhibited autophagy related gene LC3 protein expression. Blocking autophagy by 3-MA could significantly enhance myocardial hypertrophy induced by Ang Ⅱ. [Conclusion] Ang Ⅱ may induce myocardial hypertrophy by inhibiting autophagy.关键词
心肌细胞/心肌肥大/血管紧张素Ⅱ/自噬/相同基因/微管蛋白1轻链3Key words
cardiomyocyte/ hypertrophy/ angiotensin Ⅱ (Ang Ⅱ )/ autophagy related gene/ LC3( Tiny tubes related proteins 1 light chain 3)分类
医药卫生引用本文复制引用
汪海宁,刘晨,李燕辉,陈琮,孙刚,薛睿聪,董吁钢..自噬在血管紧张素Ⅱ诱导心肌细胞肥大中的作用[J].中山大学学报（医学科学版）,2012,33(4):440-443,448,5.基金项目
国家自然科学基金(81170226/H0212) （81170226/H0212）
