中山大学学报（医学科学版）2012，Vol.33Issue(4)：444-448,5.
大鼠自体原位肝移植肺组织TNF-α、IL-1β和IL-6的表达
TNF-α, IL-1β, and IL-6 Genes Transcription and Expression Level of Lung Tissue in Rats Experienced Orthotopic Autologus Liver Transplantation
摘要
Abstract
[Objective] To investigate the gene transcription and expression level of TNF-α, IL-1β, and IL-6 in lung tissue of rats experienced orthotopic autologus liver transplantation. [ Method ] Thirty healthy SPF Sprague-Dawley rats were randomly divided into five groups. According to the different times after liver reperfusion, the rats were divided into sham group (S, n = 6), 4 h after liver reperfusion group (M4, n = 6), 8h after liver reperfusion group (M8, n=6), 16 h after liver reperfusion group (M16, n=6), 24 h after liver reperfusion group (M24, n = 6). Lung tissues of the each animal in different groups were collected and sent to analyze the morphology change as well as he genes transcription and expression level of TNF-a, IL-1β, and IL-6. RT-PCR was adopted to evaluate the TNF-α, IL-1β, and IL-6 genes transcription level and ELBA method was performed to determine the TNF-a, IL-1β and IL-6 genes expression level in lung tissue. [Results] (1) Lung tissue histological analysis shown the rats in sham group exhibited the normal structure of the lung tissue and seldom inflammatory cells infiltration, while the rats received ortholopic autologus liver transplantation revealed collapse of the pulmonary structure, severely infiltration of inflammatory cells into the intra-alveolar and interstitial spaces as well as edematous interstitial space. (2) Comparing with sham group, lung water content and genes transcription level of TNF-α, IL-1β and IL-6 in M4, M8, M16 and M24 groups significantly increased (P＜ 0.05), while each of them reached the peak value at 8h after liver reperfusion. (3) Comparing with sham group, genes expression level of TNF-α, IL-1β and IL-6 in M4, M8, M16 and M24 groups significantly increased (P ＜ 0.05), TNF-α and IL-1β approached the top value at 8 h after liver reperfusion, while IL-6 reached the peak value at 16 h after liver reperfusion. [Conclusion] The genes transcription and expression level of TNF-α, IL-1β and IL-6 in lung tissue of the rats experienced orthotopic autologus liver transplantation were significantly up-regulated, which may contribute to the acute lung injury after liver transplantation.关键词
自体原位肝移植/肺/炎症因子Key words
orthotopic aulologus liver transplantation/ lung/ inflammatory cytokine分类
医药卫生引用本文复制引用
罗刚健,池信锦,张瑷兰,朱国松,黑子清..大鼠自体原位肝移植肺组织TNF-α、IL-1β和IL-6的表达[J].中山大学学报（医学科学版）,2012,33(4):444-448,5.基金项目
国家自然科学基金(30972858) （30972858）
