中山大学学报（医学科学版）2012，Vol.33Issue(4)：449-453,459,6.
Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用
Inhibition Effect of Bcl-xl Gene on Apoptosis of SY5Y Cells Induced by Sodium Nitroprusside
摘要
Abstract
[Objective] To study the effect of Bcl-xl gene on cytotoxicity of SH-SY5Y cells induced by sodium nitroprusside. [Methods] The plasmid pIRES2-EGFP/Bcl-xl was transfected into SH-SY5Y cells. Three groups were designed: A) normal control group;B) pIRES2-EGFP control group; C) plRES2-EGFP/Bcl-xl group. After SH-SY5Y cells being treated with sodium nitroprusside (50 祄ol/L), the apoptosis was valued by Hoechst33258 stain. In addition, MTT was employed to test cell viability. We also did RT-PCR and Western blotting to investigate Bcl-xl gene expression. [Results] By transfection of pIRES2-EGFP/Bcl-xl, Bcl-xl gene could effectively express in SH-SY5Y cells. Supported by MTT results, Bcl-xl gene was able to partially reduce cell death induced by sodium nitroprusside. [Conclusion] Transfection of Bcl-xl gene can increase the expression of Bcl-xl and thus lead to weaken cell death induced by NO supplied by sodium nitroprusside. This result supports that cytotoxicity induced by SNP is mainly mediated via the process of apoptosis.关键词
Bcl-xl基因/SH-SY5Y/硝普钠/凋亡/基因治疗Key words
Bcl-xl gene/ SH-SY5Y/ sodium nitroprusside/ apoptosis/ gene therapy分类
医药卫生引用本文复制引用
杨炼红,蒋龙元,蔡晓冬,刘淑琼,吴秀勤,刘军,陶恩祥..Bcl-xl基因对硝普钠诱导SY5Y细胞凋亡的抑制作用[J].中山大学学报（医学科学版）,2012,33(4):449-453,459,6.基金项目
广东省科技计划项目(2006B36004016) （2006B36004016）
广东省自然科学基金(S2011010004626) （S2011010004626）
