作物研究2012，Vol.26Issue(4)：350-354,5.DOI:10.3969/j.issn.1001-5280.2012.04.09
甘蓝型油菜叶片蛋白质双向电泳体系优化
Optimization of Two Dimensional Polyacrylamide Gel Electrophoresis System for Brassica napus Leaves Protein
摘要
Abstract
This paper compared two different protein extraction methods of Brassica napus leaves, and discussed the optimization scheme of leaves 2 - DE. The results showed that the TCA/acetone was more suitable to protein extraction compared with Tris - Cl method; using IPG of pH 4 - 7 and 12% SDS - PAGE could separate protein completely; using Coo-massie brilliant blue G250 dyeing twice could improve resolution of 2 - DE experiment results, and could separate more a-bout 2,000 protein dots, besides the results can keep stability and good repeatability.关键词
甘蓝型油菜/叶片/蛋白质/双向电泳Key words
Brassica napus/ Leaves/ Protein/ Two dimensional polyacrylamide gel electrophoresis分类
农业科技引用本文复制引用
舒佳宾,文李,官春云..甘蓝型油菜叶片蛋白质双向电泳体系优化[J].作物研究,2012,26(4):350-354,5.基金项目
湖南省研究生科研创新项目资助(CX2011B283) （CX2011B283）
中国博士后基金面上重点项目(20110490147) （20110490147）
湖南省科技计划项目(2011RS4007) （2011RS4007）
948项目(2011-G23). （2011-G23）
