作物学报2012，Vol.38Issue(5)：773-779,7.DOI:10.3724/SP.J.1006.2012.00773
抗纹枯病、根腐病的转SN1基因小麦的获得与鉴定
Development and Characterization of SN1 Transgenic Wheat Plants with Enhanced Resistance to Rhizoctonia cerealis and Bipolaris sorokiniana
摘要
Abstract
SN1 is an anti-microbial peptide in potato, and can inhibit the growth of important phytopathogens. In this study, the transformation vector of SNl gene expressing highly in monocot plants, pA25-SNl, was correctly constructed, in which SNl gene was driven by maize ubiquitin promoter. Four thousand embryo calli of Yangmai 18 were bombarded by the particle containing pA25-SN1. Among the regenerated 203 plants, 55 positive transgenic individuals were identified by PCR assay in the T0 genera-tion with a transformation frequency of 1.38%. The transgenic wheat plants from T0 to T2 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR assays. The disease responses of these positive transgenic plants were also evaluated by inoculating with Rhizoctonia cerealis and Bipolaris sorokiniana. The results showed that SN1 gene was integrated into these transgenic lines, inherited from T0 to T2, and expressed in the wheat background. The transgenic wheat plants expressing SNl displayed an enhanced resistance to R. Cerealis and 8. Sorokiniana compared with the untransformed Yangmai 18, and the enhanced resistance was inheritable.关键词
SN1基因/转基因小麦/小麦纹枯病菌/小麦根腐病菌/抗性Key words
SN1 gene/ Transgenic wheat/ Rhizoctonia cerealis/ Bipolaris sorokiniana/ Resistance引用本文复制引用
王金凤,杜丽璞,李钊,黄素萍,叶兴国,冯斗,张增艳..抗纹枯病、根腐病的转SN1基因小麦的获得与鉴定[J].作物学报,2012,38(5):773-779,7.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2008ZX08002-001,2009ZX08002-006B)资助. （2008ZX08002-001,2009ZX08002-006B）
