作物学报2012，Vol.38Issue(5)：780-790,11.DOI:10.3724/SP.J.1006.2012.00780
小麦磷饥饿前后根系蛋白质组差异表达谱建立及差异表达蛋白的鉴定
Identification of Differential Expressed Proteins Responding to Phosphorus Starvation Based on Proteomic Analysis in Roots of Wheat (Triticum aestivum L.)
摘要
Abstract
Growth inhibition caused by phosphorus (P) deficiency is a serious problem for crop production. Plants can respond defensively to this stress by modifying their metabolic pathways and root morphologies through changes of quantity of low-Pi responding genes. To better understand the adaptation mechanisms of wheat to Pi deficiency conditions, a comparative proteome analysis was conducted in this study using wheat root samples treated 7 days without (-P) and with phosphorus (+P). Among 1 144 protein spots reproducibly detected, eighty seven of which (7.6%) were differentially expressed, including those present in treatments of only in -P or +P, and up- or down-regulated in -P. Moreover, 39 of the differentially expressed proteins were revealed to be involved in various biological processes such as metabolism, cell growth and division, transcription and translation, disease and defense, signal transduction, acting as transposable elements and unclassified proteins. Taken together, our results showed that wheat responds to the Pi starvation stress through an array of changes in metabolic states of cells and genes expression, which results in the maintenance of a relative P homeostasis in plants.关键词
磷营养/蛋白质组表达谱/根系/小麦Key words
Phosphorus nutrition/ Proteomic analysis/ Root/ Triticum aestivum L.引用本文复制引用
冯万军,李振兴,郭宝健,彭惠茹,姚颖垠,倪中福,孙其信..小麦磷饥饿前后根系蛋白质组差异表达谱建立及差异表达蛋白的鉴定[J].作物学报,2012,38(5):780-790,11.基金项目
本研究由国家重点基础研究发展计划(973计划)前期项日(2007CB109000),国家杰出青年科学基金(30925023),国家自然科学基金项目(30671297)和国家高技术研究发展计划(863计划)项目(2012AA10A309)资助. （973计划）
