作物学报2012，Vol.38Issue(5)：808-813,6.DOI:10.3724/SP.J.1006.2012.00808
疣粒野生稻泛素结合酶基因的全长cDNA序列克隆与分析
Cloning and Analysis on Full-Length Cdna Sequence of Ubiquitin-Conjugating Enzyme Gene from Oryza meyeriana Baill
摘要
Abstract
Based on the bioinformatic analysis of the ESTs from the subtraction cDNA library of Oryza meyeriana, we cloned one of the ESTs which has the homolog function of ubiquitin-conjugating enzyme and obtained a full-length cDNA sequence of ubiquitin-conjugating enzyme gene by RACE, and named it 0mE2 (Oryza meyeriana Baill ubiquilin-conjugating enzyme). This full-length cDNA sequence has 917 bp including 528 bp open reading frame encoding a protein of 175 amino acids, with a predicted molecular mass of 19.3 kD and pI of 9.32. 0mE2 protein, which had significant amino sequence identity and similarity with ubiquitin-conjugating enzyme from other species contained the conserved sequence and cysteine residue in the active sites of ubiquitin-conjugating family. 0mE2 had three trans-membrane domains. It is predicted that 0mE2 protein is a kind of ubiquitin-conjugating protein and transmembrane protein. The expression of 0mE2 in Oryza meyeriana was induced by Xanthomonas campestris pv. Oryzae. Therefore 0mE2 gene which was first found in wild rice may participate in the signal transduction and the resistance response in Oryza meyeriana.关键词
疣粒野生稻/泛素结合酶/序列分析Key words
Oryza meyeriana Baill./ Ubiquitin-conjugating enzyme/ Sequence analysis引用本文复制引用
蒋春苗,程在全,黄兴奇,付坚,余腾琼,钟巧芳,李定琴,殷富有,张敦宇,王玲仙..疣粒野生稻泛素结合酶基因的全长cDNA序列克隆与分析[J].作物学报,2012,38(5):808-813,6.基金项目
本研究由国家自然科学基金项目(31160067,31000746),国家公益性行业科研专项(201003021),云南省重点应用基础研究项目(2008C004)和云南省自然科学基金项目(2010CD098)资助. （31160067,31000746）
