作物学报2012，Vol.38Issue(5)：814-819,6.DOI:10.3724/SP.J.1006.2012.00814
连续回交对消除农杆菌介导转化引起水稻体细胞变异的影响
Effect of Successive Backcrossing on Eliminating Somaclonal Variation Caused by Agrobacterium-Mediated Transformation in Rice
摘要
Abstract
Somaclonal variation caused by Agrobacterium-mediated transformation influences some agronomic traits of trans-genic plants. As a result, a large number of To regenerated plants are required in the development of transgenic crop. Here, we successively backcrossed transgenic rice (Oryza saliva L.) to progenitor variety and evaluated agronomic performance of progeny, with the objective of eliminating somaclonal variation and recovering agronomic traits of transgenic parent. Three transgenic lines possessing different insect-resistance genes from Bacillus thuringiensis (Bt) were used as donor parents. They were backcrossed successively to the progenitor variety, and the individuals of each BCnF1 generation were then selfed twice, with selection for insect-resistance. BCnF3 homozygous lines, which were selected by a germination assay, caused an insect mortality of 100% in laboratory bioassay. In the field evaluation, these lines showed obviously higher yield than the donor parents and no significant differences from the progenitor variety in most agronomic traits. These results indicate that successive backcrossing can to a large extent recover agronomic traits of transgenic rice and reduce the workload required in transgenic breeding program.关键词
连续回交/体细胞变异/农杆菌介导转化/转基因水稻/抗虫性/农艺性状Key words
Successive backcross/ Somaclonal variation/ Agrobacterium-mediated transformation/ Transgenic rice/ Insect-resistance/ Agronomic trait引用本文复制引用
杨宙,陈浩,唐微,林拥军..连续回交对消除农杆菌介导转化引起水稻体细胞变异的影响[J].作物学报,2012,38(5):814-819,6.基金项目
本研究由国家转基因生物新品种培育重大专项(2008ZX08001-001)资助. （2008ZX08001-001）
