作物学报2012，Vol.38Issue(5)：820-828,9.DOI:10.3724/SP.J.1006.2012.00820
运用关联分析定位栽培大豆蛋白11S、7S组分的相关基因位点
Identification of QTLs for Glycinin (11S) and β-Conglycinin (7S) Fractions of Seed Storage Protein in Soybean by Association Mapping
摘要
Abstract
Glycinin (11S) and p-conglycinin (7S) are major components of seed storage protein in soybean, which play important roles in the functionality of seed protein. In the present study, association mapping was used to map the QTLs (quantitative trait loci) for glycinin (11S) and P-conglycinin (7S) fractions. One hundred and sixty-six accessions from Chinese soybean minicore collection were genotyped with 134 selected simple-sequence repeat (SSR) markers. The storage protein of each accession was separated by SDS-PAGE method, and gels were scanned for individual subunits of 11S and 7S by ImageQuant TL software. The association analysis between SSR loci and protein subunit components was performed with TASSEL GLM (general linear model) and MLM (mixed linear model) programs. The results showed that, both in 2008 and 2010, fourteen SSR loci associated with the protein subunit components were screened out by GLM program, and five SSR (Satt234, Satr595, Sat_062, Satt583, and Satt291) loci by MLM program, respectively. The high variation of 7S subunits was the main reason that caused 11S/7S ratio variance Fewer loci were detected to be associated with the protein subunits whose phenotypic variations were higher, which might be due to the more recombination incidents during the evolution of the related genes. Therefore, the LD decay distances of these genes were short, some QTLs could not be detected with limited SSR markers. The results of this study are meaningful for the marker assisted selection breeding of soybean varieties with higher protein quality.关键词
栽培大豆[Glycine max (L.) Merr.]/11S亚基/7S亚基/SSR/关联分析Key words
Cultivated soybean [Glycine max (L.) Merr.]/ Glycinin (11S)/ β-conglycinin (7S)/ Simple-sequence repeat (SSR)/ Association mapping引用本文复制引用
简爽,文自翔,李海朝,袁道华,李金英,张辉,叶永忠,卢为国..运用关联分析定位栽培大豆蛋白11S、7S组分的相关基因位点[J].作物学报,2012,38(5):820-828,9.基金项目
本研究由国家自然科学基金项日(30971818,30771360),国家转基因生物新品种培育重大专项(2011ZX08004-003),河南省创新型科技人才队伍建设工程(094100510004)和郑州市创新型科技人才队伍建设工程(096SYJH14103)资助. （30971818,30771360）
