作物学报2012，Vol.38Issue(5)：914-920,7.DOI:10.3724/SP.J.1006.2012.00914
斑茅割手密杂种后代真实性鉴定及遗传分析
Identification and Genetic Analysis of Hybrid from Cross between Erianthus arundinacius (Retz.) Jesws.and Saccharum spontaneum L.
摘要
Abstract
Hybridization between different populations is the most common means to enrich genetic variation. Studies on genetic differentiation and characterizations of hybrid are crucial to protect and evaluate genetic resources. In this paper, the hybrid from Erianthus arundinacius (Retz.) Jesws. X Saecharum spontaneum L. Were analyzed using SSR and SRAP markers. The results showed that the hybrid GXAS07-6-1 was the true hybrid identified by using three pairs of SSR primers and five pairs of SRAP primers. A total of 1 095 DNA bands were amplified by 71 pairs of selective SRAP primers. Among 1 095 genetic loci, 800 were polymorphic. Therefore, the average polymorphic ratio was 73.0% and each pair of SRAP primers yielded 11 polymorphic loci on average. The genetic relationship between the hybrid and its parents analyzed with software NTSYS-pc2.10e showed that the genetic similarity coefficient was 0.474 between the parents, while 0.696 between the hybrid and the female parent and 0.752 between the hybrid and the male parent, suggesting that the hybrid GXAS07-6-1 was more inherited from male parent.关键词
斑茅/割手密/杂种鉴定/SSR/SRAPKey words
Erianthus arundinacius (Retz.) Jesws./ Saccharum spontaneum L./ Hybrid identification/ SSR/ SRAP引用本文复制引用
刘昔辉,张革民,李杨瑞,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆..斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920,7.基金项目
本研究由国家自然科学基金项目(31101195,30971837,30860150),国家科技支撑计划项目(2007BAD30B00),国家现代农业产业技术体系建设专项(CARS-20-3),广西自然科学基金项目(0991009Z,2010GXNSFB013027,2011GXNSFF018002)和广西农科院基金项目(200906Z,200923,G2010005,G2009008,G2009010,桂农科2011YT01,桂农科2012YZ10)资助. （31101195,30971837,30860150）
