作物学报2012，Vol.38Issue(7)：1178-1186,9.DOI:10.3724/SP.J.1006.2012.01178
水稻抗纹枯病QTL qSB-9TQ和抗条纹叶枯病基因Stv-bi的聚合育种
Rice Pyramiding Breeding Using Sheath Blight Resistance QTL qSB-9TQ and Stripe Disease Resistance Gene Stv-bi
摘要
Abstract
Indica rice cultivar Teqing and japonica rice cultivar Zhendao 88 were used as the donor parents with the sheath blight (SB) resistance QTL qSB-9TQ and the resistance gene Stv-bi, respectively. Commercial rice japonica cultivars, Wuyujing 3 and Wujing 15, were used as the receipt parents to cross and backcross with the donor parents, respectively. Flanked polymorphic markers to the two loci were developed and employed to select the target loci via marker assisted selection (MAS) in each back-cross and self-cross progeny. Till backcross generation BC4F1, pyramiding was performed by crossing the selected individuals with different integression genes. At F3 generation, the pyramiding lines with homozygosity at both gene loci were obtained. Results of disease resistance evaluation indicated that both pyramiding lines were resistant to stripe disease, and increased resistance to SB with a reducing SB sating of 1.1-1.6 in Wuyujing 3 background and 0.8-1.4 in Wujing 15 background based on a "0-9" SB rating scale. Combining the results of resistance phenotype at the low backcrossing generation, we thought the markers developed in this study were efficient in selecting the two genes by MAS. Some questions associated with SB resistance breeding and pyramiding breeding via MAS were discussed.关键词
分子标记辅助选择/纹枯病/条纹叶枯病/聚合育种Key words
Marker assisted selection/ Sheath blight/ Stripe disease/ Pyramiding breeding引用本文复制引用
陈宗祥,左示敏,张亚芳,朱俊凯,王龙平,冯凡,马玉银,潘学彪..水稻抗纹枯病QTL qSB-9TQ和抗条纹叶枯病基因Stv-bi的聚合育种[J].作物学报,2012,38(7):1178-1186,9.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2006AA10Z165,2006AA10A103),国家转基因生物新品种培育科技重大专项(2009ZX08001-014B),国家自然科学基金项目(30900883)和江苏高校作物优势学科建设工程项目资助. （863计划）
