作物学报2012，Vol.38Issue(7)：1232-1239,8.DOI:10.3724/SP.J.1006.2012.01232
烟草细胞质雄性不育系及其保持系的花蕾差异蛋白质分析
Proteomic Analysis of Bud Differentiation between Cytoplasmic Male-Sterile Line and Maintainer in Tobacco
摘要
Abstract
To explore the molecular genetic mechanisms of cytoplasmic male sterility (CMS) in tobacco, we studied the differential proteins between the CMS line and its maintainer. Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) technique was used to separate the protein spots while gels were stained with the Coomassie Blue G-250. The difference between protein maps of bud from cytoplasmic male-sterile line MSK326 and maintainer line K326 was analyzed with PDQuest image software. Matrix-assisted laser-adsorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technique was used to obtain the peptide mass of differentially expressed protein spots. The Mascot software was used to search the protein database NCBlnr to identify those spots interested. A total of 365 protein spots were detected within Mr 14.4-97.6 kD and pH 4-7. Seven protein spots appeared in the protein map of maintainer line K326 but absent in that of CMS line MSK326. Which were identified as ribulose-l,5-bisphosphate carboxylase/oxygenase, polyphenol oxidase, patatin homolog, and PSI 9 kD protein. It was inferred that the male sterility of MSK326 might be related to energy metabolism turbulence, abnormality in male organ development, deficient nutrition supply and immunity, and inhibition of energy transfer.关键词
烟草/细胞质雄性不育/花蕾/差异蛋白质/双向凝胶电泳Key words
Tobacco/ Cytoplasmic male sterility/ Bud/ Differential protein/ 2-DE引用本文复制引用
祁建民,马红勃,徐建堂,陈美霞,周东新,王涛,陈顺辉..烟草细胞质雄性不育系及其保持系的花蕾差异蛋白质分析[J].作物学报,2012,38(7):1232-1239,8.基金项目
本研究由中国烟草总公司重点项目(110201002006)资助. （110201002006）
