作物学报2012，Vol.38Issue(8)：1347-1353,7.DOI:10.3724/SP.J.1006.2012.01347
水稻早衰突变体esl2的遗传分析及基因定位
Genetic Analysis and Gene Mapping of Early Senescence Leaf Mutant esl2 in Rice
摘要
Abstract
An early senescence leaf mutant temporarily designated as esl2 was discovered in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by ethyl methane sulfonate (EMS). The mutant kept normal at seedling stage and showed etiolated senescence leaves from booting sage to the maturity, especially at tip and margin of leaf blade. By contrast with the wild type, photosynthetic pigment contents decreased significantly at both booting stage and heading stage, superoxide dismutase (SOD) activity and soluble protein contents decreased significantly, while the activities of peroxidase (POD) and catalase (CAT), and contents of reactive oxygen species (ROS), malondialdehyde (MDA) and praline increased significantly at heading stage in the esl2 mutant. The assay of Transmission Electronic Microscope (TEM) observation was performed and demonstrated that the most cells appeared irregular and void with endolysis and disrupted organdies at senescence part of the mutational leaf, concomi-tantly, abnormal chloroplast appeared and showed dissolved membrane, indistinct grana, disorganized lamellar structure and incorrect thylakoid. Genetic analysis suggested that the mutational trait was controlled by one nuclear recessive gene. Xinong I A was crossed with the esl2 and 1 005 mutational F2 single plants were used for gene mapping. Finally, Esl2 locus was mapped between SSR marker RM17122 and swu4-13 on the chromosome 4 with physical distance of 244 kb. This result provides a foundation of Esl2 gene cloning by map-based strategy as well as its functional analysis.关键词
水稻(Oryza sativa L.)/早衰/基因定位Key words
Rice (Oryza sativa L.)/ Early senescence/ Gene mapping引用本文复制引用
徐芳芳,桑贤春,任德勇,唐彦强,胡宏伟,杨正林,赵芳明,何光华..水稻早衰突变体esl2的遗传分析及基因定位[J].作物学报,2012,38(8):1347-1353,7.基金项目
本研究由中央高校基本科研业务费专项(XDJK2009B019)和国家自然科学基金项目(31071072)资助. （XDJK2009B019）
