作物学报2012，Vol.38Issue(8)：1369-1377,9.DOI:10.3724/SP.J.1006.2012.01369
山农01-35×藁城9411重组自交系遗传图谱构建及粒重QTL分析
Construction of Genetic Map and Analysis of QTLs for Grain Weight Using a RIL Population Derived from Shannong 01-35 × Gaocheng 9411
摘要
Abstract
The objectives of this study were to construct a new linkage map of wheat using a recombinant inbred line (RIL) population derived from the cross between Shannong 01-35 and Gaocheng 9411 and locate quantitative trait loci for thousand-grain weight (TGW) using this map. The RIL population, consisting of 182 lines, was obtained via single-seed descendent method until the F8 generation. The genetic map consisted of one TaGW2-CAPS, 59 SSR, and 442 DArT markers in 29 linkage groups, including 54 novel markers (44 DArT markers and 10 SSRs) assigned into 18 linkage groups. The total genetic length of the map was 4084.5 cM with an average interval distance of 8.13 cM. The 182 RILs and their parents were grown in four environments from 2008 to 2010, and QTLs associated with TGW were identified using mixed linear model based on both separated and joint environments. A total of seven QTLs were detected including three QTLs (QGW4B-7, QGW5B-20, and QGW6A-29) commonly found using both methods. Three major QTLs, I.e., QGW4B-7, QGW5B-12, and QGW6A-29, exhibited phenotypic contributions higher than 10%. These results suggest that the QTLs detected by using the successfully constructed genetic map are valuable in molecular marker-assisted selection in wheat.关键词
小麦/遗传图谱/粒重/QTLKey words
Wheat/ Genetic map/ Grain weight/ QTL引用本文复制引用
师翠兰,郑菲菲,陈建省,韩淑晓,王永瑞,田纪春..山农01-35×藁城9411重组自交系遗传图谱构建及粒重QTL分析[J].作物学报,2012,38(8):1369-1377,9.基金项目
本研究由国家重点基础研究计划(973计划)项目(2009BC118301)和国家自然科学基金项目(31171554)资助. （973计划）
