作物学报2012，Vol.38Issue(8)：1416-1424,9.DOI:10.3724/SP.J.1006.2012.01416
两个新水稻Dwarf18基因强等位突变体的表型分析及分子鉴定
Phenotypic Analysis and Molecular Characterization of Two Allelic Mutants of the Dwarf18 Gene in Rice
摘要
Abstract
We identified two strong dwarf mutants from Nipponbare by EMS mutagenesis, designated as s2-9 and sl-146a. The plant height of these two mutants was 26.3% and 19.2% of that of Nipponbare at the mature stage. They also had wider and dark green leaf at seedling stage. The GA3 treatment of seedling and a-amylase activity analysis in endosperm showed that the mutated gene was involved in GA biosynthesis. Fine mapping showed that the mutant phenotype was tightly linked with the D18 locus. There was a single nucleotide substitution at the 3'-splicing site between the first intron and the 2nd exon in s2-9, whereas there was a single nucleotide substitution in the 2nd exon in sI-146a, which caused a premature stop codon. Semi quantitative RT-PCR revealed that transcript of the D18 gene was up-regulated in sl-146a as compared to WT, but was not detected in s2-9.关键词
水稻/矮秆突变体/Dwarf18/赤霉素/精细定位Key words
Rice/ Dwarf mutant/ Dwarf18/ GA/ Fine mapping引用本文复制引用
侯雷,袁守江,尹亮,赵金凤,万国峰,张文会,李学勇..两个新水稻Dwarf18基因强等位突变体的表型分析及分子鉴定[J].作物学报,2012,38(8):1416-1424,9.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2011CB100200)资助. （973计划）
