作物学报2012，Vol.38Issue(8)：1538-1543,6.DOI:10.3724/SP.J.1006.2012.01538
用基因枪法获得转异天南星基因aha抗蚜虫小麦
Generation of Aphid Resistant Transgenic Wheat with aha from Arisaema heterophyllum by Particle Bombardment
摘要
Abstract
Agglutinin is a class of mannose-binding protein, which has detrimental effect on aphids and other pests. In this study, the vector harboring aha cloned from Arisaema heterophyllum directed by rice Rubisco small subunit promoter (rbcS) was constructed. By co-transformed with pAHC20 that harbors bar selection gene, the aha gene was transferred into wheat variety Kenong 199 via bombardment. After induction, regeneration, two rounds of selection and with the conformation by PCR analysis, 42 transgenic plants with foreign aha gene were obtained, average co-transformation efficiency of 2.41%. According to PCR analysis, the segregation of the T1 plants was basically consistent with Mendel's separation. Aphid resistance bioassay was carried out using eight randomly selected transgenic lines by multiple discrimination method. One high-resistant and three low-resistant transgenic lines were identified, accounting for 44.4% of the tested materials. This study has laid a basis for application of aha gene in development of aphid resistant transgenic wheat.关键词
小麦/植物凝集素/Rubisco小亚基启动子/基因枪转化/共转化/抗虫性Key words
Wheat/ Agglutinin/ rbcS promoter/ Particle bombardment/ Co-transformation/ Insect resistance引用本文复制引用
张彦,喻修道,唐克轩,夏兰琴..用基因枪法获得转异天南星基因aha抗蚜虫小麦[J].作物学报,2012,38(8):1538-1543,6.基金项目
本研究由国家转基因生物新品种培育重大专项(2011ZX08002-001)资助. （2011ZX08002-001）
