植物学报2012，Vol.47Issue(3)：286-291,6.DOI:10.3724/SP.J.1259.2012.00286
五唇兰合子与原胚的分离
Isolation of Zygotes and Proembryos in Doritis pulcherrima
摘要
Abstract
The isolation of zygotes and proembryos would provide invaluable material for the study of plant fertilization and embryogenesis. We isolated zygotes and proembryos by manual microdissection from Doritis pulcherrima embryo sacs with only one layer of integument cells after pretreatment combining enzymatic maceration with shaking to the ovules. The enzymatic maceration was with 0.7%-1.3% cellulase, 0.6%~1.0% pectinase, and 10% mannitol, pH5.8, for one-half to 3 hours. Suspensor haustorium was discovered on the micropylar end from early proembryo to maturing spherical proembryo stages. The successful isolation of zygotes and proembryos in 0. pulcherrima provides a novel approach to study embryogenesis and is a valuable experimental tool for further detailed research at the cellular and molecular levels.关键词
五唇兰/显微解剖/原胚/胚柄吸器/合子Key words
Doritis pulcherrima/ microdissection/ proembryo/ suspensor haustorium/ zygote引用本文复制引用
伍成厚,李冬妹,田惠桥..五唇兰合子与原胚的分离[J].植物学报,2012,47(3):286-291,6.基金项目
广东省科技计划(No.2007B020801007)和广州市科技计划(No.11A63030233) （No.2007B020801007）
