植物学报2012，Vol.47Issue(4)：395-404,10.DOI:10.3724/SP.J.1259.2012.00395
大麦黄矮病毒运动蛋白是RNA沉默抑制因子
The Movement Protein of Barley Yellow Dwarf Virus is a Suppressor of RNA Silencing
摘要
Abstract
The genome of barley yellow dwarf virus (BYDV), a member of the Luteoviridae family, contains 6 open reading frames (ORFs). We cloned the 6 ORFs into plasmid of pWEIMING101 and electroporated them into Agrobacte-rium tumefaciens for transient coexpression of BYDV ORFs and GFP in Nicotiana benthamiana line 16c, a transgenic GFP plant. Northern blot analysis revealed the movement protein (MP) encoded by BYDV-PAV ORF4 as a suppressor of both local and systemic GFP RNA silencing and RNA silencing triggered by double-stranded GFP RNA. The fluorescent intensity was associated with steady state levels of GFP mRNAs and its degradation product of siRNAs. The nuclear localization signal in the N terminus of the MP and amino acid 5 and 6 were the functional domain and motif, respectively, essential for inhibiting RNA silencing. Transient expression of BYDV-MP alone in infiltrated tissue resulted in cell death.关键词
大麦黄矮病毒/运动蛋白/RNA沉默/抑制因子Key words
barley yellow dwarf virus/ movement protein/ RNA silencing/ suppressor引用本文复制引用
申永梅,刘国富,杨纪君,霍晓辉,曹雪松..大麦黄矮病毒运动蛋白是RNA沉默抑制因子[J].植物学报,2012,47(4):395-404,10.基金项目
国家自然科学基金(No.30870109) （No.30870109）
