中国中西医结合杂志2012，Vol.32Issue(7)：970-974,5.
增免抑瘤颗粒剂抗卵巢癌荷瘤小鼠皮下移植瘤血管生成作用的实验研究
Anti-angiogenic Effects of Zengmian Yiliu Granule on Ovarian Carcinoma Xenograft
摘要
Abstract
Objective To investigate the anti-angiogenic effects and mechanisms of Zengmian Yiliu Granule (ZMYLG) on ovarian carcinoma xenograft. Methods The SKOV3 ovarian carcinoma bearing mouse model was established. The tumor-bearing mice were randomly divided into the control group, the paclitaxel group, the high, medium, and low dose ZMYLG group, 8 in each group. The medication was lasted for ten days. The mi-crovessel density (MVD) in the xenograft was calculated by the method of using cell membrane differentiation antigen 34 (CD34) antibody marking new vascular endothelial cells. The protein and mRNA expressions of vascular endothelial growth factor (VEGF)and its receptor fetal liver kinase-1 (FLK-1), hypoxia inducible factor-lα (HIF-1α) in the tumor were determined using immunohistochemical assay and RT-PCR. Results The MVD of ovarian carcinoma xenografts in the paclitaxel group, the high, medium, and low dose ZMYLG group obviously decreased , showing statistical difference when compared with the control group ( P < 0. 01, P < 0. 05). Each ZMYLG dose group could down-regulate the protein and mRNA expressions of VEGF, FLK-1, and HIF-1α (P< 0. 01, P<0. 05). Conclusions ZMYLG could inhibit neogenesis of tumor vessels. Its mechanisms might be associated with down-regulating the expression of HIF-1cc, modifying the hypoxic state, inhibiting the expressions of VEGF and FLK-1, and exerting its anti-angiogenic effects.关键词
增免抑瘤颗粒剂/卵巢癌/血管生成/缺氧诱导因子-1αKey words
Zengmian Yiliu Granule/ ovarian carcinoma/ angiogenesis/ hypoxia inducible factor-1α引用本文复制引用
胡欣欣,张勤华,齐聪,李久现..增免抑瘤颗粒剂抗卵巢癌荷瘤小鼠皮下移植瘤血管生成作用的实验研究[J].中国中西医结合杂志,2012,32(7):970-974,5.基金项目
上海市重点学科建设项目(No.S30303) （No.S30303）
上海市卫生局中医药科研基金项目(No.2008X001A) （No.2008X001A）
上海市科委中药现代化项目(No.06DZ19704) （No.06DZ19704）
