中国中西医结合杂志2012，Vol.32Issue(8)：1032-1037,6.
慢性乙型肝炎肝郁脾虚证和脾胃湿热证患者的差异表达基因研究初探
Study on the Differential Gene Expressions of Chronic Hepatitis B Patients of Gan Depression Pi Deficiency Syndrome and Pi-Wei Damp-heat Syndrome
摘要
Abstract
Objective To explore the differential gene expressions of chronic hepatitis B (CHB) of Gan depression Pi deficiency syndrome (GDPDS) and Pi-Wei damp-heat syndrome (PWDHS). Methods The ulnar venous blood was withdrawn from healthy subjects (26 cases), patients of GDPDS (35 cases) and PWDHS (34 cases) on an empty stomach. The total RNA was extracted using Trizol method. The differentia! genes were de-tected using Aglient expression profile chip and screened using randomized variance model. The results were an-alyzed using GO, Pathway, GeneBank, NCBI, and Geneontology. Results There were 125 differential genes between CHB patients of GDPDS and those of PWDHS (including 66 up-regulated genes and 59 down-regulated genes), mainly involving in functions of transmembrane transport, response to selenium ion, and regulation of calcium ion-dependent exocytosis. The signal pathway participated in mainly includes cell adhesion molecules, calcium ion signaling pathway, leukocyte trans-endothelial migration. We present gene co-expression networks to find 9 interactions among genes (LOC340508, HIST2H2BE, MPL, FLJ22536, TUBA8, NT5M, EGFL7, PT-PRF, TSPAN33), which were mainly involved in immune response, cell growth, DNA damage, signal transduc-tion, inflammatory reaction, and so on. Conclusions The differential expression genes existed between CHB pa-tients of GDPDS and those of PWDHS, indicating that Chinese medicine syndrome classification has its own ba-sis for gene expression profile. The genomics research method is expected to provide an objective basis for Chi-nese medicine syndrome typing.关键词
慢性乙型肝炎/肝郁脾虚证/脾胃湿热证/差异表达基因,基因芯片Key words
chronic hepatitis B/ Gan depression Pi deficiency syndrome/ Pi-Wei damp-heat syndrome/ differential expression gene/ gene chip引用本文复制引用
杨婵娟,刘宏伟,王丽春,张传涛,冯全生,范昕建..慢性乙型肝炎肝郁脾虚证和脾胃湿热证患者的差异表达基因研究初探[J].中国中西医结合杂志,2012,32(8):1032-1037,6.基金项目
国家科技重大专项资助项目——病毒性肝炎中医证候生物学技术平台研究(No.2009ZX10005-020) （No.2009ZX10005-020）
