中国中西医结合杂志2012，Vol.32Issue(8)：1064-1068,5.
银杏叶提取物对衰老大鼠前额叶皮层和海马炎性细胞因子及胶质细胞超微结构的影响
Effects of Ginkgo biloba Extract 50 on Inflammatory Cytokines and Glia Cell Ultrastructures in the Prefrontal Cortex and Hippocampus of Aging Rats
摘要
Abstract
Objective To study the effects of Ginkgo biloba extract 50 (GBE50) on inflammatory cytokines and glia cell injury in the prefrontal cortex and hippocampus of aging rats and its probable mechanism. Methods Totally 45 mate SD rats were randomly divided into 4 groups, i. e. the normal control group (n = 12), the mode! group (n= 11), the low dose GBE50 group (n=10), and the high dose GBE50 group (n=12). The aging rat model was intraper-itoneally injected with D-galactose to establish the aging model for 42 days. Starting from the 22nd day of modeling, rats in the low dose GBE50 group and the high dose GBE50 group were administered by gastrogavage with 75 mg/kg and 150 mg/kg respectively. The protein contents and mRNA expressions of IL-1β IL-6, and TNF-α in the prefrontal cortex and hippocampus of rats were detected by radioimmunoassay and Real-time fluorescence quantitative PCR assay re-spectively. The ultrastructural changes of glia cells in the hippocampal CA1 region were observed by transmission elec-tron microscope. Results The protein contents and mRNA expressions of IL-1β and TNF-a in the prefrontal cortex and the hippocampus of aging rats obviously increased when compared with the normal control group (P<0.05, P<0.01). The content of IL-6 in the hippocampus of aging rats obviously decreased (P <0. 01). Compared with the model group, the protein content and mRNA expression of IL-β in the prefrontal cortex and the hippocampus were obviously down-regulated in the low and high dose GBE50 groups. The content of TNF-α in the prefrontal cortex was obviously down-regulated in the low and high dose GBE50 groups, the content of TNF-α in the hippocampus was obviously down-regulated in the low dose GBE50 group (P<0.05, P<0. 01). The content of IL-6 in the prefrontal cortex of the low dose GBE50 group was up-regulated. The content of IL-6 in the hippocampus of the high dose GBE50 group was also up-regulated. The mRNA expression of IL-6 in the prefrontal cortex of the low and high dose GBE50 groups obviously in-creased (P<0. 05, P<0.01). Low and high dose GBE50 showed obvious recovery on the ulirastructural damage of glia cells in the hippocampal CA1 region. Conclusions GBE50 showed inhibitive effects on the inflammatory reaction of nerves of aging rats. Its mechanism might be possibly correlated with its regulatory effects on the cytokines in the pre-frontal cortex and the hippocampus, as well as the ultrastructures of glia cells in the prefrontal cortex and hippocampus to some degree.关键词
衰老/前额叶皮层/海马/细胞因子/胶质细胞/银杏叶提取物Key words
aging/ the prefrontal cortex/ the hippocampus/ cytokine/ glia cell/ Ginkgo biloba extract 50引用本文复制引用
贺改英,张志雄,徐颖,董献文,王星禹..银杏叶提取物对衰老大鼠前额叶皮层和海马炎性细胞因子及胶质细胞超微结构的影响[J].中国中西医结合杂志,2012,32(8):1064-1068,5.基金项目
上海市自然科学基金资助项目(No.09ZR1432100) （No.09ZR1432100）
上海市教委重点学科资助项目(No.J50301) （No.J50301）
上海市教委预算内科研项目(No.2010JW04) （No.2010JW04）
