中国中西医结合杂志2012，Vol.32Issue(8)：1069-1072,4.
葛根素对大鼠脑缺血再灌注侧皮质区细胞凋亡及p-Akt(Ser473)表达的影响
Effects of Puerarin on the Neurocyte Apoptosis and p-Akt (Ser473) Expressions In Rats with Cerebral Ischemia/Reperfusion Injury
摘要
Abstract
Objective To observe the effects of puerarin (Pue) on the neurocyte apoptosis and the p-Akt (Ser473) expression in the ischemic penumbra of rats with cerebral ischemia/reperfusion (I/R). Methods The 48 Sprague-Dawley rats were randomly divided into four groups, i. e. , the sham-operation group, the I/R group, the Pue treatment group, and the Pue + LY294002 treatment group (Pue +LY), 12 in each group. The cerebral I/R rat model was established by Longa's suture method. Pue and Pue + specific P13K kinase inhibitor, i. e. , LY294002 were administered. The score of the neurological deficit was estimated 1 h followed by 24 h reperfu-sion. The infarct volume was measured using TTC staining. The number of apoptotic neurons were detected u-sing Tunel method. The expressions of p-Akt (Ser473) was detected using immunohistochemical assay, and the images were analyzed. Results The score of the neurological deficit decreased more obviously, the number of apoptosis decreased more significantly, the expressions of p-Akt (Ser473) increased more significantly in the Pue group than in the I/R group (all P<0. 05). The score of the neurological deficit increased more obviously, the number of apoptosis increased more significantly, the expression of p-Akt (Ser473) decreased more significantly in the Pue + LY group than in the Pue group (all P<0. 05). Conclusion Pue reduced the apoptosis of neurocytes and had protective effects against cerebral I/R injury possibly through activating the PI3K/Akt signaling pathway.关键词
葛根素/脑缺血再灌注/细胞凋亡/p-Akt(Ser473)Key words
puerarin/ cerebral ischemia/reperfusion/ apoptosis/ p-Akt (Ser473)引用本文复制引用
韩江全,于奎营,何敏,李向荣,胡泳涛,林冬融,卢俊江..葛根素对大鼠脑缺血再灌注侧皮质区细胞凋亡及p-Akt(Ser473)表达的影响[J].中国中西医结合杂志,2012,32(8):1069-1072,4.基金项目
广东省中医药局科研课题(No.2008286) （No.2008286）
珠海市重点学科项目资助(No.200880) （No.200880）
