中国中西医结合杂志2012，Vol.32Issue(8)：1088-1094,7.
大承气汤含药血清对内毒素刺激的人支气管上皮细胞表达CAV-1、eNOS及NF-κB的影响
Effects of Dachengqi Decoction Containing Serum on the Expressions of Caveolin-1, eNOS, and NF-κB in Lipopolysaccharide Stimulated Human Bronchial Epithelial Cells
摘要
Abstract
Objective To observe the effects of Dachengqi Decoction (DD) containing serum on the ex-pressions of caveolin-1 (CAV-1), endothelial nitric oxide synthase (eNOS), and nuclear transcription factor-kappa B (NF-kB) in lipopolysaccharide (LPS) stimulated human bronchial epithelial cells (HBECs). Methods The DD and the DD containing serum were prepared. The in vitro cultured HBECs were randomly divided into 7 groups, i. e., the normal serum control group, the LPS intervention group, the low dose DD serum containing group, the mid-dle dose DD serum containing group, the high dose DD serum containing group, the Western medicine control group, the vehicle serum control group. The effects of DD containing serum at different doses on the mRNA and protein expressions of CAV-1, eNOS, and NF-kB were detected using methyl thiazolyl tetrazolium (MTT) colorime-try, Real-time PCR, immuocytochemical assay, and Western blot. Results The mRNA and protein expressions of CAV-1, eNOS, and NF-kB at the basic levels were detected in the HBECs of the normal serum control group. After stimulated by LPS, the mRNA and protein expressions of CAV-1, eNOS, and NF-kB increased more significantly in the LPS intervention group than in the normal serum control group (P<0.01), while DD containing serums at dif-ferent doses all could suppress the mRNA and protein expressions of CAV-1, eNOS, and NF-kB. Conclusion DD containing serum could inhibit the expressions of CAV-1, eNOS, and NF-kB in LPS stimulated HBECs.关键词
大承气汤/内毒素/人支气管上皮细胞/小凹蛋白-1/内皮型一氧化氮合酶/核因子-κBKey words
Dachengqi Decoction/ lipopolysaccharide/ human bronchial epithelial cell/ caveolin-1/ endothelial nitric oxide synthase/ nuclear transcription factor-kappa B引用本文复制引用
杨胜兰,金阳,沈霖,刘建国,樊琼,薛卡明,黄璐,吴嫣然..大承气汤含药血清对内毒素刺激的人支气管上皮细胞表达CAV-1、eNOS及NF-κB的影响[J].中国中西医结合杂志,2012,32(8):1088-1094,7.基金项目
国家自然科学基金面上项目(No.30873225) （No.30873225）
湖北省卫生厅资助项目(No.2010Z-Y20) （No.2010Z-Y20）
