中国中西医结合杂志2012，Vol.32Issue(9)：1223-1226,4.
高脂及劳倦伤脾大鼠有机阴离子转运肽oatp4a1 mRNA及蛋白表达
Organic Anion Transporting Polypeptide (oatp4a1) mRNA and Protein Expressions in High Fat and Overfatigue Impairing Pi Rats
摘要
Abstract
Objective To study organic anion transporting polypeptide (OATP) superfamily member 4a1 (oatp4a1) mRNA expression in the Pi deficiency model rats, thus exploring its mechanism for transporting and transforming the dampness. Methods Six SD rats of SPF grade were used to prepare over-fatigue impairing Pi model. Another 12 SD rats were randomly divided into the blank control group and the high fat diets group, 6 in each. The special binding tube was used for the over-fatigue impairing Pi model group on the odd day, 3 h each time. Then the rats were forced to swim in the cold water (10 ℃ ±1℃) for 7 min on the even day, for 2 succes-sive weeks. Rats in the model group and the blank control group were granulatedly fed for 12 weeks, while rats in the high fat group were fed with high fat diet for 12 weeks. All rats were free to take food and drink water. The mRNA and protein expressions of oatp4a1 were detected in the Fei, Pi, Gan, Shen, Wei, Xiaochang, and Dachang using Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR)and Western blot. Results Rats in the model group were idled together with lousy defecate and coarse skin. They ate and drank less, and lost body weight (P<0. 05). They were consistent with clinical manifestations of Pi deficiency syndrome, indica-ting that the over-fatigue impairing Pi animal model was successfully established. Rats in the high fat group star-ted to have poor appetite and languish spirit, move lazily and addict to sleep, have coarse, dark, and colorless hair 9 weeks later, indicating phlegm dampness syndrome. Compared with the blank control group, the average body weight increased in the high fat group at the 9th week ( P<0. 05). The oatp4a1 mRNA expressed in the Fei, Pi, Gan, Shen, Wei, Xiaochang, and Dachang. There was no statistical difference in the oatp4a1 mRNA expression among all tissues (P>0. 05). The oatp4a1 mRNA expressions were higher in the Fei and Shen of the high fat group than in the Gan (P <0. 05). Conclusions oatp4a1 might be one of the basic substances in the transportation and transformation of phlegm dampness. Of them, Fei, Shen, and Dachang might play important roles in the transportation and transformation of phlegm dampness.关键词
湿浊/脾虚大鼠/有机阴离子转运肽oatp4a1Key words
dampness/ Pi deficiency rat/ organic anion transporting polypeptide 4a1引用本文复制引用
董霄,潘爱珍,孙保国,周厚明,陈泽雄,张诗军..高脂及劳倦伤脾大鼠有机阴离子转运肽oatp4a1 mRNA及蛋白表达[J].中国中西医结合杂志,2012,32(9):1223-1226,4.基金项目
国家自然科学基金资助项目(No.81072806) （No.81072806）
国家“十一五”科技重大专项资助项目(No.2009ZX10005-016) （No.2009ZX10005-016）
广东省自然科学基金资助项目(No.9151008004000030) （No.9151008004000030）
