中国中西医结合杂志2012，Vol.32Issue(9)：1227-1232,6.
黄芪桔梗汤及黄芪黄连汤对克罗恩病大鼠肺与结肠ICAM-1蛋白及mRNA的影响
Effects of Huangqi Jiegeng Decoction and Huangqi Huanglian Decoction on the Protein and mRNA Expressions of ICAM-1 in the Lung and Colon of Rats with Crohn's Disease
摘要
Abstract
Objective To observe the effects of Huangqi Jiegeng Decoction (HJD) and Huangqi Huangli-an Decoction (HHD) on the intercellular adhesion molecule-l (ICAM-1) content in the lung and colon of rats with Crohn's disease (CD). Methods Totally 56 rats were used to establish the CD rat model using TNBS water so-lution/absolute alcohol enema (with the model successful rate of 63. 0%). Seven rats randomly selected from 35 successfully modeled rats and from the normal group were recruited as the model group and the normal group be-fore intervention. The rest 28 successfully modeled rats were randomly divided into the model group, the Western medicine group (treated with salazosulfapyridine at 0. 4 g/kg), the HJD group (20. 5 g/kg), and the HHD group (20. 8 g/kg), 7 in each group. Another 7 normal rats were recruited as the normal group. Equal volume of pure water was given to rats in the normal group and the model group by gastrogavage, twice daily, for 3 successive weeks. The protein and mRNA expressions of ICAM-1 in the lung and colon tissues were determined before and after intervention using Western blot and RT-PCR. Results Compared with the normal control group, the protein and mRNA levels of ICAM-1 in the colon tissue, and the mRNA level of ICAM-1 in the lung tissue increased ( P< 0. 01, P <0. 05). After intervention the protein and mRNA levels of ICAM-1 in the colon tissue and the lung tissue increased( P<0. 01) in the model group. Compared with the model group at the same time point, the protein and mRNA levels of ICAM-1 in the colon tissue and the lung tissue decreased in each medication group after interven-tion (P<0. 01, P<0. 05). Compared with the Western medicine group, the protein level of ICAM-1 in the lung tissue decreased more significantly in the HJD group (P<0. 05). The protein level of ICAM-1 in the colon tissue also decreased in the HHD group (P<0. 05). Conclusions HHD and HJD both could down-regulate the over-ex-pressed ICAM-1 in the lung and colon tissues of CD rats. HHD was prominent in inhibiting the adherence of co-Ionic inflammatory cells and attenuating local immunopathological injury. HJD was prominent in attenuating inflam-mation and injury in the lung, and preventing pulmonary fibrosis.关键词
克罗恩病/黄芪桔梗汤/黄芪黄连汤/内皮细胞黏附分子-1/肺损伤Key words
Crohn's disease/ Huangqi Jiegeng Decoction/ Huangqi Huanglian Decoction/ intercellular adhesion molecule-l/ lung injury引用本文复制引用
杨舒,王新月,杨雪,景姗,朱陵群,王硕仁..黄芪桔梗汤及黄芪黄连汤对克罗恩病大鼠肺与结肠ICAM-1蛋白及mRNA的影响[J].中国中西医结合杂志,2012,32(9):1227-1232,6.基金项目
国家重点基础研究发展计划(973计划)资助项目(No.2009CB522705) （973计划）
